Bone Anabolic Effects of Soluble Si: In Vitro Studies with Human Mesenchymal Stem Cells and CD14+ Osteoclast Precursors

Stem Cells International
João Costa-RodriguesMaria H Fernandes

Abstract

Silicon (Si) is indispensable for many cellular processes including bone tissue metabolism. In this work, the effects of Si on human osteogenesis and osteoclastogenesis were characterized. Human mesenchymal stem cells (hMSC) and CD14+ stem cells, as osteoblast and osteoclast precursors, were treated with a wide range of Si concentrations, covering the physiological plasma levels. Si promoted a dose-dependent increase in hMSC proliferation, differentiation, and function, at levels similar to the normal basal plasma levels. Additionally, a decrease in the expression of the osteoclastogenic activators M-CSF and RANKL was observed. Also, Si elicited a decrease in osteoclastogenesis, which became significant at higher concentrations, as those observed after meals. Among the intracellular mechanisms studied, an upregulation of MEK and PKC signalling pathways was observed in both cell types. In conclusion, Si appears to have a direct positive effect on human osteogenesis, at basal plasma levels. On the other hand, it also seemed to be an inhibitor of osteoclastogenesis, but at higher concentrations, though yet in the physiological range. Further, an indirect effect of Si on osteoclastogenesis may also occur, through a downregulation o...Continue Reading

References

Nov 10, 1972·Science·E M Carlisle
Sep 1, 1993·Calcified Tissue International·M HottP J Marie
Dec 28, 1999·Journal of Inorganic Biochemistry·D M ReffittJ J Powell
Mar 25, 2000·The American Journal of Clinical Nutrition·R JugdaohsinghJ J Powell
Oct 6, 1972·Nature·K Schwarz, D B Milne
Feb 19, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ravin JugdaohsinghJonathan J Powell
Jun 2, 2006·Journal of Biomedical Materials Research. Part a·C M BotelhoW Bonfield
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Qingxiao ZhaoYi-Ping Li
Jan 17, 2008·Current Opinion in Otolaryngology & Head and Neck Surgery·Donald J Annino, Laura A Goguen
Apr 15, 2008·Archives of Biochemistry and Biophysics·Koichi Matsuo, Naoko Irie
Apr 22, 2008·Archives of Biochemistry and Biophysics·H Kalervo Väänänen, Tiina Laitala-Leinonen
Apr 29, 2008·Journal of Clinical Pathology·H K DattaS S Varanasi
Nov 17, 2009·Journal of Cellular Biochemistry·João Costa-RodriguesMaria H Fernandes
Feb 16, 2010·Acta Biomaterialia·Daniel Arcos, María Vallet-Regí
May 27, 2010·The Journal of Biological Chemistry·Liza J Raggatt, Nicola C Partridge
Jun 25, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Pedro S GomesMaria H Fernandes
Aug 21, 2010·Calcified Tissue International·Matthias WiensWerner E G Müller
Feb 25, 2011·Acta Biomaterialia·Ming-You ShieHsien-Chang Chang
Apr 5, 2011·Dental Materials : Official Publication of the Academy of Dental Materials·A Pelaez-VargasF J Monteiro
Aug 5, 2011·Journal of Cellular Biochemistry·João Costa-RodriguesMaria H Fernandes
Oct 3, 2012·Dental Materials : Official Publication of the Academy of Dental Materials·A CarvalhoF J Monteiro
Nov 16, 2012·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J W Nieves
Jan 12, 2013·Biological Trace Element Research·Eun-Jin KimMi-Kyeong Choi
Apr 10, 2013·Biomedical Materials·Chengtie Wu, Jiang Chang
Jun 14, 2013·International Journal of Endocrinology·Charles T PriceJoshua R Langford
Sep 11, 2013·Acta Biomaterialia·Živko MladenovićMaria Ransjö
Nov 10, 2013·BioMed Research International·Ana Claudia M RennoNivaldo Antonio Parizotto
Apr 8, 2015·Journal of Biomedical Materials Research. Part a·Robert J FriederichsSerena M Best

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Citations

Apr 4, 2019·Journal of Cellular Physiology·Sara RochaJoão Costa-Rodrigues
Mar 21, 2019·Current Drug Targets·Azam HosseinzadehSaeed Mehrzadi
Sep 7, 2019·Current Stem Cell Research & Therapy·Ying Chen, Liling Tang
Sep 18, 2020·Biochemical Society Transactions·Eric MadsenDavid H Kohn
Apr 6, 2021·Journal of Biomedical Materials Research. Part a·Catarina MagnussonMaria Ransjö

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Methods Mentioned

BETA
Magnetic Cell Sorting
ELISA
light microscopy

Software Mentioned

ImageJ

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