PMID: 9549611Apr 29, 1998Paper

Bone cell behavior on Matrigel-coated Ca/P coatings of varying crystallinities

Journal of Biomedical Materials Research
S H MaxianJ P Zawadsky

Abstract

Rat calvarial cell mitogenic behavior was investigated on various biomaterials coated with Matrigel, a basement membrane matrix containing growth factors. Low (20-40%) and high (70-90%) crystallinity hydroxyapatite (rHA and cHA), rough titanium (Ti), and tissue culture polystyrene (TP) surfaces were compared. Surface chemistry and calcium resorption of HA coatings, alkaline phosphatase activity (APA), and growth of cells were measured for Matrigel-coated and uncoated surfaces at 2, 7, and 14 days. Gene expression for four noncollagenous bone-related proteins (osteonectin, osteopontin, alkaline phosphatase, and osteocalcin) was also investigated by reverse transcription and polymerase chain reaction up to 28 days. Ca concentration in incubating solutions increased with time for the two types of HA coatings and was always greater for rHA than cHA. Surface chemistry and coating dissolution rates were not affected by the presence of Matrigel or cells throughout the study. APA of cells on the two HA-coated surfaces was comparably enhanced in the presence of Matrigel and was greater than on Ti surfaces. Only HA surfaces showed an increased APA of cells with time in the presence of Matrigel. Cell growth peaked at 7 days and was greate...Continue Reading

References

Jun 1, 1991·Journal of Biomedical Materials Research·D A PuleoR Bizios
Feb 1, 1990·Journal of Biomedical Materials Research·M GrégoireJ Menanteau
Jan 1, 1987·Annual Review of Cell Biology·G R Martin, R Timpl
Jan 1, 1985·Journal of Cellular Biochemistry·H K KleinmanM DuBois-Dalcq
Aug 1, 1995·Journal of Biomedical Materials Research·J E HulshoffJ A Jansen
Aug 1, 1995·The Journal of Bone and Joint Surgery. American Volume·D R SumnerJ O Galante
Feb 1, 1995·Journal of Biomedical Materials Research·J L OngL C Lucas
Nov 1, 1994·Journal of Biomedical Materials Research·S H MaxianM G Dunn
Dec 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D J BaylinkS Mohan
Aug 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S PitaruN Savion
Nov 1, 1993·Journal of Cellular Physiology·K TraianedesD M Findlay
Apr 1, 1993·Journal of Biomedical Materials Research·K Gomi, J E Davies
Aug 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L K McCauleyR T Franceschi
May 1, 1996·Endocrinology·Y Mikuni-TakagakiS Saito
Sep 1, 1996·Journal of Biomedical Materials Research·K KieswetterB D Boyan
Nov 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R KornuR L Smith
Jul 1, 1994·Journal of Applied Biomaterials : an Official Journal of the Society for Biomaterials·W R GombotzP A Puolakkainen

❮ Previous
Next ❯

Citations

Dec 28, 2011·Journal of Materials Science. Materials in Medicine·Ronaldo de Oliveira LomelinoJosé Mauro Granjeiro
Oct 20, 2005·Journal of Bioscience and Bioengineering·T SuzukiM Toriyama
Jun 19, 2010·The Journal of Oral Implantology·Rahul BholaTimothy Ohno
Jan 5, 2002·Journal of Biomedical Materials Research·D BeckerK-W Wenzel
Jun 24, 2008·Acta Biomaterialia·Yu-Peng LuJian-Hua Wang
Apr 26, 2008·Journal of Biomedical Materials Research. Part a·E A dos SantosK Anselme
Jul 19, 2003·Clinical Oral Implants Research·Petra J ter BruggeJohn A Jansen
Jul 10, 2007·Journal of Biomedical Materials Research. Part a·S Sh HomaeigoharA Yari Sadi
Aug 13, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Heemin KangShyni Varghese
Nov 15, 2000·Journal of Biomedical Materials Research·S SofiaD L Kaplan
Nov 19, 2003·Journal of Biomedical Materials Research. Part a·R ShuL R McCabe
Feb 17, 2005·Journal of Biomedical Materials Research. Part a·Jianwei XieLaura R McCabe
Aug 11, 2007·Journal of Biomedical Materials Research. Part a·M W LaschkeM D Menger
Jul 13, 2005·Journal of Biomedical Materials Research. Part a·Euler A dos SantosGloria A Soares
Aug 11, 2005·Journal of Biomedical Materials Research. Part a·S Sh HomaeigoharM Hosseinalipour

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.