Bone-forming peptide-2 derived from BMP-7 enhances osteoblast differentiation from multipotent bone marrow stromal cells and bone formation

Experimental & Molecular Medicine
Hyung Keun KimTaek Rim Yoon

Abstract

Strategies for efficient osteogenic differentiation and bone formation from stem cells would have clinical applications in treating nonunion fracture healing. Many researchers have attempted to develop adjuvants as specific stimulators of bone formation for therapeutic use in patients with bone resorption. Therefore, development of specific stimulators of bone formation has therapeutic significance in the treatment of osteoporosis. To date, investigations of the mature forms of bone morphogenetic proteins (BMPs) have focused on regulation of bone generation. However, we previously identified new peptides from the immature precursor of BMP, and further analysis of these proteins should be performed. In this study, we identified a new peptide called bone-forming peptide-2 (BFP-2), which has stronger osteogenic differentiation-promoting activity than BMP-7. BFP-2 treatment of multipotent bone marrow stromal cells (BMSCs) induced expression of active alkaline phosphatase. In addition, BFP-2 enhanced CD44 and CD51 expression levels and increased Ca2+content in BMSCs. Moreover, radiography at 8 weeks revealed that animals that had received transplants of BFP-2-treated BMSCs showed substantially increased bone formation compared with ...Continue Reading

References

Oct 20, 2000·Clinical Orthopaedics and Related Research·G A DahirG Balian
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marcelo Paez-PeredaEduardo Arzt
Sep 20, 2005·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·Chung-Hwan ChenGwo-Jaw Wang
Jul 28, 2007·Annals of the New York Academy of Sciences·Petros D DamoulisDavid L Kaplan
Feb 24, 2009·Biomaterials·Diederik H R KempenWouter J A Dhert
May 26, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Liang ChenZhong-Liang Deng
Jul 17, 2012·Biomaterials·Hyung Keun KimTaek Rim Yoon
Mar 30, 2013·Journal of Dental Research·C G WilsonR T Franceschi
Apr 9, 2013·Maturitas·Franz JakobHeike Walles
Sep 23, 2014·Biomaterials·Hyun-Ji ParkSeung-Woo Cho

❮ Previous
Next ❯

Citations

Oct 17, 2019·Journal of Biomedical Materials Research. Part a·Muhammad QasimNae Yoon Lee
Jul 30, 2019·Journal of Food Biochemistry·Chang-Bum Ahn, Jae-Young Je
Feb 26, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ariane G FurlanGerhard Sengle
Apr 15, 2021·Molecular Therapy. Methods & Clinical Development·Marjolein M J CaronTim J M Welting
Jul 5, 2021·Cellular Signalling·Laura-Marie A ZimmermannGerhard Sengle
Aug 14, 2020·Journal of Agricultural and Food Chemistry·Zhe XuMing Du
Apr 8, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yanchao CuiXueliang Zhang

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
Assay
PCR
fluorescence-activated cell sorting
flow cytometry

Software Mentioned

Image
Dicom Works
SPSS
CellQuest
Pro Plus

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.