Bone marrow connexin-43 expression is critical for hematopoietic regeneration after chemotherapy

Cell Communication & Adhesion
Cynthia A PresleyJ A Cancelas

Abstract

Contact between bone marrow (BM) hematopoietic stem cells (HSC) and osteoblast/stromal (OS) cells has been shown to be crucial in the regulation of hematopoiesis. However, very little is known about the regulatory mechanisms of direct cell-to-cell communication in the hematopoietic microenvironment. Gap junction channels (connexons) are formed by polypeptides (connexins) arranged in hexamers and represent the best described intercellular communication system. Connexin-43 (Cx43) is expressed by BM OS cells and has been associated with the cadherin/beta -catenin signaling pathway, recently reported as relevant in the OS/HSC interaction at the stem cell niche. Here, we employed an inducible gene-targeted murine approach to study the role of Cx43 in HSC proliferation and differentiation in vivo. Mx-Cre/Cx43+/+ and Mx-Cre/Cx43flox/flox littermates have been analyzed after gene deletion induced in vivo by the interferon-inducer poly (I)-poly (C), generating control (Cx43+) and Cx43-deficient (Cx43-/-) mice. After one week, Cx43+ and Cx43-/- mice were treated with 5-fluorouracil (5-FU). Cx43 expression in Cx43-/- BM was markedly reduced (> 90%) as analyzed on day +14 post-5-FU treatment. Cx43 deficiency did not induce a significant ch...Continue Reading

References

Jun 1, 1988·Clinical Nuclear Medicine·G D RieserA R Cordell
Mar 1, 1972·Cell and Tissue Kinetics·J Gidali, L G Lajtha
Jan 1, 1970·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·H CroizatM Tubiana
Sep 8, 1995·Science·R KühnK Rajewsky
Aug 1, 1996·Journal of Bioenergetics and Biomembranes·R D Veenstra
Aug 7, 1998·Molecular Biology of the Cell·F LecandaR Civitelli
Apr 30, 2003·Nature·Tannishtha ReyaIrving L Weissman
Oct 24, 2003·Nature·L M CalviD T Scadden
Apr 20, 2004·Cardiovascular Research·Goran Söhl, Klaus Willecke
Jul 19, 2005·Nature Medicine·Jose A CancelasDavid A Williams
Sep 13, 2005·Development·Ann M FlennikenJanet Rossant

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Citations

Apr 20, 2011·Nature Immunology·Michael D Milsom, Andreas Trumpp
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eri Taniguchi IshikawaJose A Cancelas
Aug 5, 2008·The Journal of Biological Chemistry·Arlene J Siller-JacksonJean X Jiang
Apr 10, 2009·Stem Cells and Development·Brynjar FossOystein Bruserud
Apr 30, 2011·Circulation Research·Benjamin F RemoGlenn I Fishman
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Nellie KalchevaGlenn I Fishman
Mar 13, 2014·FEBS Letters·Christina L GrekGautam S Ghatnekar
May 8, 2010·Expert Opinion on Therapeutic Targets·Mustapha Kandouz, Gerald Batist
Jun 30, 2012·Journal of Thrombosis and Haemostasis : JTH·M P Mahaut-Smith
Mar 5, 2015·Journal of Cellular Biochemistry·Carolina C DiasEdgar J Paredes-Gamero
Dec 31, 2014·Cell and Tissue Research·Trond Aasen
Apr 5, 2012·Cell Transplantation·Flávio Henrique Paraguassú-BragaAdriana Bonomo
Jul 27, 2007·The Journal of Membrane Biology·Maria Lúcia Zaidan Dagli, Francisco Javier Hernandez-Blazquez
May 7, 2013·Nature Medicine·Daniel LucasPaul S Frenette
Jan 30, 2020·International Journal of Molecular Sciences·Abhishek K Singh, Jose A Cancelas
Jun 4, 2020·International Journal of Molecular Sciences·Cristina PanuzzoDaniela Cilloni
Dec 22, 2016·Frontiers in Physiology·Pui WongGary Tse
Sep 19, 2017·Frontiers in Immunology·María Alejandra GleisnerAndrés Tittarelli
Mar 31, 2018·Current Stem Cell Reports·Nafiisha GenetKaren K Hirschi

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