Bone Marrow-Derived and Elicited Peritoneal Macrophages Are Not Created Equal: The Questions Asked Dictate the Cell Type Used.

Frontiers in Immunology
Cheryl M ZajdMichelle R Lennartz

Abstract

Macrophages are a heterogeneous and plastic population of cells whose phenotype changes in response to their environment. Macrophage biologists utilize peritoneal (pMAC) and bone marrow-derived macrophages (BMDM) for in vitro studies. Given that pMACs mature in vivo while BMDM are ex vivo differentiated from stem cells, it is likely that their responses differ under experimental conditions. Surprisingly little is known about how BMDM and pMACs responses compare under the same experimental conditionals. While morphologically similar with respect to forward and side scatter by flow cytometry, reports in the literature suggest that pMACs are more mature than their BMDM counterparts. Given the dearth of information comparing BMDM and pMACs, this work was undertaken to test the hypothesis that elicited pMACs are more responsive to defined conditions, including phagocytosis, respiratory burst, polarization, and cytokine and chemokine release. In all cases, our hypothesis was disproved. At steady state, BMDM are more phagocytic (both rate and extent) than elicited pMACs. In response to polarization, they upregulate chemokine and cytokine gene expression and release more cytokines. The results demonstrate that BMDM are generally more r...Continue Reading

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Citations

Jun 9, 2020·Frontiers in Oncology·Erica J Hoffmann, Suzanne M Ponik
May 22, 2021·Frontiers in Cell and Developmental Biology·Stefania CrippaMaria Ester Bernardo
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang ZhouZhimin Zhao

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Methods Mentioned

BETA
flow cytometry
transgenic
lavage
PCR
transfecting
ELISA
Assay
Polymerase Chain Reaction

Software Mentioned

FlowJo
Regeneron
GPower
Multiplex®
Minitab

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