Bone marrow-derived keratinocytes are not detected in normal skin and only rarely detected in wounded skin in two different murine models

Experimental Hematology
Qingyuan FanJonathan C Vogel

Abstract

Because the ability of bone marrow-derived cells (BMDCs) to repopulate tissues and the possible mechanisms of repopulation remain controversial, we used two distinct murine models to determine whether BMDCs can repopulate epidermal keratinocytes during either steady-state homeostasis or after tissue injury. The accessibility of skin keratinocytes makes it an excellent tissue to assess BMDC repopulation. In the two murine models, BMDCs from either male homologous B6, 129S Rosa26 mice that constitutively express ss-galactosidase or male hemizygote C57 BL/6-Tg(ACTbEGFP)1Osb/J mice expressing enhanced green fluorescent protein were transplanted via tail vein injection into control lethally irradiated (9.5 Gy) congenic female recipients and the percentage of keratinocytes derived from the transplanted BMDCs, both with and without wounding, was carefully determined. Analysis of bone marrow, thymus, spleen, and lymph nodes confirmed complete engraftment of donor BMDCs 6 months post-bone marrow transplantation. However, during steady-state homeostasis, bone marrow-derived keratinocytes could not be detected in the epidermis. In a skin wound-healing model, the epidermis contained only rare bone marrow-derived keratinocytes (< 0.0001%) b...Continue Reading

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Citations

Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Katsuto TamaiYasufumi Kaneda
Dec 18, 2012·Immunotherapy·Alya Abdul-WahabJohn A McGrath
Oct 28, 2008·Burns : Journal of the International Society for Burn Injuries·Suzanne ReaMark W Fear
Dec 18, 2007·Journal of Dermatological Science·Daiki RokunoheDaisuke Sawamura
Sep 2, 2009·Experimental Dermatology·Katherine LauArdeshir Bayat
Jun 22, 2010·Oral Diseases·J VerstappenJ W Von den Hoff
Jun 28, 2012·International Wound Journal·Yu WangZhao-Fan Xia
Aug 4, 2011·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Wei LiuZhen Gao
Sep 4, 2009·Journal of Cellular and Molecular Medicine·Yannick LandryChristian M Beauséjour
Feb 13, 2013·Journal of Biophotonics·Benedikt W GrafStephen A Boppart
May 8, 2010·Dermatologic Clinics·Maija KiuruAngela M Christiano
Jan 10, 2013·Journal of Wound Care·C J Lewis
Jul 28, 2019·Experimental Dermatology·Shadi KhalilArturo P Saavedra

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