Bone Marrow-Derived Mesenchymal Stem Cells Exert Diverse Effects on Different Macrophage Subsets

Stem Cells International
Bin ChenFuhua Yan

Abstract

Mesenchymal stem cells (MSCs) and their secreted molecules have shown great potential for tissue regeneration and the treatment of inflammation and autoimmune diseases. However, they can also be associated with therapeutic failure or even side effects. Possible causes for this could include the state of the stem cells themselves and the influence of the local microenvironment, wherein macrophages play important roles. As such, we utilized conditioned medium from bone marrow-derived MSCs (MSC-CM) and studied its effect on different macrophage subsets. Effects on macrophage proliferation, apoptosis, polarization, and phagocytosis were determined, and it was discovered that MSC-CM had no significant effect on macrophage proliferation but inhibited M0 macrophage apoptosis and marginally induced M1 macrophage apoptosis. MSC-CM was shown to reduce CD80 expression on the surface of M1 macrophages. Moreover, it promoted and inhibited CD163 expression on the surface of M0 and M1 macrophages, respectively. However, MSC-CM tended to initially promote CD163 expression on M2 macrophages but inhibited expression of this marker after additional incubation time. Unlike MSCs, MSC-CM had no significant effect on the expression of TNF-α and IL-10...Continue Reading

References

Aug 22, 2006·Journal of Molecular and Cellular Cardiology·Xiaohong LiShuguang Lin
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
May 2, 2009·Journal of Leukocyte Biology·Andrew J FleetwoodJohn A Hamilton
Mar 20, 2010·Molecular Immunology·Hanne Van GorpHans J Nauwynck
Sep 22, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Moïra FrançoisJacques Galipeau
May 2, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Derek C LaceyJohn A Hamilton
Sep 12, 2012·Current Opinion in Lipidology·Vicente AndrésCarlos Silvestre-Roig
Apr 23, 2013·The American Journal of Pathology·Lizhi He, Alexander G Marneros
Aug 27, 2013·Journal of the American College of Cardiology·Tamar Ben-MordechaiJonathan Leor
Oct 9, 2013·The Journal of Experimental Medicine·Stephen J JenkinsJudith E Allen
Jun 30, 2015·The American Journal of Pathology·Amitava DasSashwati Roy
Mar 18, 2016·Immunity·Thomas A Wynn, Kevin M Vannella
May 14, 2016·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Zhensen ZhuEdward E Tredget
Dec 14, 2016·The New England Journal of Medicine·Peter W MarksRobert M Califf
Jan 14, 2018·Biomaterials·Jukka PajarinenStuart B Goodman

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Citations

Jul 11, 2019·Stem Cells Translational Medicine·Golnar ShojaatiJames L Funderburgh
Aug 23, 2019·Archives of Toxicology·Vanessa HartwigSteven Dooley
Nov 30, 2019·Stem Cells International·June Seok HeoHyun Ok Kim
Jun 18, 2020·World Journal of Stem Cells·Guo-Ping XuEr-Man Chen

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Methods Mentioned

BETA
Assay
flow cytometry
Flow

Software Mentioned

GraphPad Prism

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