Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene

In Vitro Cellular & Developmental Biology. Animal
Qianxu LiuXiaoming Zhao

Abstract

Bone marrow-derived mesenchymal stem cells can differentiate into a variety of adult cells. Brain-derived neurotrophic factor (BDNF) is briefly active during differentiation and induces mesenchymal stem cells to differentiate into nerve cells. In this study, we cloned human BDNF to generate a recombinant pcDNA3.1(-)-BDNF vector and transfected the vector into bone marrow-derived mesenchymal stem cells. We selected these cells with Geneticin-418 to obtain BDNF-BMSCs, which were induced with retinoic acid to obtain induced BDNF-BMSCs. The transfected cells displayed the typical morphology and surface antigen profile of fibroblasts and were observed to express clusters of differentiation 29, 44, and 90 (observed in matrix and stromal cells), but not clusters of differentiation 31, 34, and 45 (observed in red blood cells and endothelial cells), via flow cytometry. Enzyme-linked immunosorbent assays showed that transfected bone marrow-derived mesenchymal stem cells secreted more BDNF than non-transfected bone marrow-derived mesenchymal stem cells. Immunocytochemistry and real-time reverse transcription polymerase chain reaction analysis showed that non-induced BDNF-BMSCs maintained a higher proliferative capacity and expressed highe...Continue Reading

References

Mar 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Z R ShiY S Kim
Jun 16, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·K LeeJ D Mosca
Jun 22, 2002·Nature·Yuehua JiangCatherine M Verfaillie
Mar 29, 2003·Learning & Memory·Bai Lu
Jun 28, 2003·Anatomical Science International·Tatsunori Seki
Aug 2, 2003·Blood·Erica L HerzogDiane S Krause
Aug 5, 2003·Journal of Cellular Biochemistry·Melody BaddooDonald G Phinney
Jul 28, 2004·Stem Cells·Joanna E GroveDiane S Krause
Dec 2, 2004·Frontiers in Neuroendocrinology·Lucia Tapia-ArancibiaSandor Arancibia
Apr 6, 2005·Transplantation Proceedings·C LangeH C Fiegel
Feb 17, 2007·Acta Pharmacologica Sinica·Feng-Ze WangLi-Hong Ye
Sep 18, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Eeva-Mari JouhilahtiJuha Peltonen
Nov 3, 2009·Biochemical and Biophysical Research Communications·Yajing WangXiaodong Han
May 29, 2010·Development, Growth & Differentiation·Yang BiTingyu Li
Sep 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·M L Alves da SilvaN M Neves

❮ Previous
Next ❯

Citations

Feb 17, 2017·In Vitro Cellular & Developmental Biology. Animal·Azim ParandakhMohammad-Mehdi Khani
Nov 17, 2020·Molecular and Cellular Biochemistry·Jiaqiang DengShumin Yu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry
transfection
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

- Gate
Paint
Premier
SPSS

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.