Bone marrow-derived mesenchymal stem cells assembled with low-dose BMP-2 in a three-dimensional hybrid construct enhances posterolateral spinal fusion in syngeneic rats

The Spine Journal : Official Journal of the North American Spine Society
Tao HuHee-Kit Wong

Abstract

The combination of potent osteoinductive growth factor, functional osteoblastic cells, and osteoconductive materials to induce bone formation is a well-established concept in bone tissue engineering. However, supraphysiological dose of growth factor, such as recombinant human bone morphogenetic protein 2 (rhBMP-2), which is necessary in contemporary clinical application, have been reported to result in severe side effects. We hypothesize that the synergistic osteoinductive capacity of low-dose bone morphogenetic protein 2 (BMP-2) combined with undifferentiated bone marrow-derived stromal cells (BMSCs) is comparable to that of osteogenically differentiated BMSCs when used in a rodent model of posterolateral spinal fusion. A prospective study using a rodent model of posterolateral spinal fusion was carried out. Thirty-six syngeneic Fischer rats comprised the patient sample. Six groups of implants were evaluated as follows (n=6): (1) 10 µg BMP-2 with undifferentiated BMSCs; (2) 10 µg BMP-2 with osteogenic-differentiated BMSCs; (3) 2.5 µg BMP-2 with undifferentiated BMSCs; (4) 2.5 µg BMP-2 with osteogenic-differentiated BMSCs; (5) 0.5 µg BMP-2 with undifferentiated BMSCs; and (6) 0.5 µg BMP-2 with osteogenic-differentiated BMSCs. O...Continue Reading

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Citations

Oct 22, 2016·The Spine Journal : Official Journal of the North American Spine Society·Bret H CloughCarl A Gregory
Nov 9, 2016·Journal of Orthopaedics·Adam E M EltoraiAlan H Daniels
May 19, 2017·Tissue Engineering. Part B, Reviews·Rui M DuarteJorge Correia-Pinto
Jul 25, 2019·Tissue Engineering. Part a·Ling LiuHee-Kit Wong
Apr 22, 2018·Current Osteoporosis Reports·Divya Rani BijukumarMathew T Mathew
Jun 16, 2021·Operative Neurosurgery·Joshua L GolubovskyMichael P Steinmetz

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