Bone Marrow-Derived Mesenchymal Stem Cells Promoted Cutaneous Wound Healing by Regulating Keratinocyte Migration via β2 -Adrenergic Receptor Signaling

Molecular Pharmaceutics
Jiahui HuoXiaobing Fu

Abstract

Mesenchymal stem cells (MSCs) play an important role in cutaneous wound healing; however, the functional mechanisms involved in the healing process are poorly understood. A series of studies indicate that keratinocytes that migrate into the wound bed rely on an epithelial-mesenchymal transition (EMT)-like process to initiate re-epithelialization. We therefore examined whether bone marrow-derived MSCs (BMSCs) could affect biological behavior and induce EMT-like characteristics in the human epidermal keratinocytes (HEKs) and in the immortalized human keratinocyte cell line HaCaT cells, and we investigated the signaling pathways of BMSC-mediated phenotypic changes. By assessing the expression of EMT-related markers including E-cadherin, α-SMA, and Snail family transcription factors by β2-adrenergic receptor (β2-AR) blockage using ICI-118,551, a β2-AR selective antagonist, or β2-AR small interfering RNA (siRNA), we showed an involvement of β2-AR signaling in the induction of EMT-like alterations in human keratinocytes in vitro. β2-AR signaling also affected collective and individual cell migration in human keratinocyte cell lines, which was attenuated by administration of ICI-118,551. Treating the cells with BMSC-conditioned media ...Continue Reading

References

Jan 12, 1996·The Journal of Biological Chemistry·K BaltenspergerC C Malbon
Jan 24, 2003·American Journal of Physiology. Cell Physiology·Chih-Ko YehMichael S Katz
May 22, 2004·Stem Cells·Joachim OswaldCarsten Werner
Jul 21, 2004·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Robert D GalianoGeoffrey C Gurtner
Sep 25, 2004·Journal of Cellular Physiology·Pierre SavagnerLaurie G Hudson
Oct 27, 2004·Experimental Cell Research·Daryle DePianto, Pierre A Coulombe
Mar 2, 2005·Nature Reviews. Immunology·Ronald Glaser, Janice K Kiecolt-Glaser
Nov 18, 2005·Lancet·Vincent Falanga
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anil K SoodSteven W Cole
May 17, 2006·Clinical and Experimental Pharmacology & Physiology·Bruna R SouzaAndréa M A Costa
Jan 11, 2007·Journal of Cellular Biochemistry·Derek C RadiskyMina J Bissell
Apr 25, 2007·Cell and Tissue Research·Andrea T BadilloKenneth W Liechty
Jul 20, 2007·Journal of Cellular and Molecular Medicine·Virgil PăunescuErhard Seifried
Aug 22, 2007·Neuroimmunomodulation·Lisa M ChristianJanice K Kiecolt-Glaser
Oct 2, 2007·Dermatologic Clinics·Raja K SivamaniR Rivkah Isseroff
Nov 3, 2007·The Journal of Clinical Investigation·Andrzej Slominski
Apr 1, 2008·Journal of Cellular and Molecular Medicine·Olivera StojadinovicMarjana Tomic-Canic
May 21, 2008·International Journal of Cosmetic Science·P QuatresoozG E Piérard
Jul 17, 2008·Nature Reviews. Molecular Cell Biology·Matthias Schäfer, Sabine Werner
Aug 8, 2008·The Journal of Investigative Dermatology·Donna F KusewittLaurie G Hudson
Aug 12, 2008·International Journal of Medical Sciences·Daniel ChesikJacques De Keyser
Sep 23, 2008·Experimental Dermatology·Alexander StoffDavid T Curiel
Mar 27, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Bruna Romana-SouzaAndréa Monte-Alto-Costa
Jul 21, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Eun Young LeeJong-Hyuk Sung
Aug 1, 2009·Journal of Dermatological Science·Laurie G HudsonDonna F Kusewitt
Nov 12, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Chandan K SenMichael T Longaker
Dec 1, 2009·Cell·Jean Paul ThieryM Angela Nieto
Nov 10, 2010·Expert Opinion on Biological Therapy·Timothy J MyersAnna Spagnoli
Dec 21, 2010·Journal of Dermatological Science·Motonobu Nakamura, Yoshiki Tokura
Feb 1, 2011·Stem Cells·Vladislav VolarevicMiodrag Stojkovic
Nov 26, 2011·Dermatology : International Journal for Clinical and Investigative Dermatology·Anabel S de la Garza-RodeaDirk W van Bekkum
Apr 3, 2012·Annual Review of Biochemistry·Qi Qun Tang, M Daniel Lane
May 3, 2012·Critical Care Nursing Clinics of North America·Jean-Philippe Gouin, Janice K Kiecolt-Glaser
May 9, 2012·Journal of Burn Care & Research : Official Publication of the American Burn Association·Cynthia E WeberPaul C Kuo

❮ Previous
Next ❯

Citations

Dec 13, 2019·Journal of Cellular and Molecular Medicine·Yiwen ZhouQingfeng Li
Nov 20, 2020·International Journal of Biological Macromolecules·Parinaz Nezhad-MokhtariNima Abdyazdani
Sep 12, 2021·Nature Reviews. Cancer·Anabel EckerlingShamgar Ben-Eliyahu
Mar 14, 2021·Archivum Immunologiae Et Therapiae Experimentalis·Wen-Yan TangXue-Qun Luo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.