PMID: 9161546Jan 1, 1997Paper

Bone marrow in patients with pseudarthrosis. A study of progenitor cells by in vitro cloning

Revue de chirurgie orthopédique et réparatrice de l'appareil moteur
P Hernigou, F Beaujean

Abstract

The recent observations that osteoblasts develop from a stem cell found in the medullary stroma suggest that, as already suspected in classical histological studies, many consolidation mechanisms are based on bone marrow. Since cells characteristics can now be studied in tissue culture, it has become possible to investigate the activity of the bone marrow in non union. The working hypothesis for this study was: if the bone marrow plays a part in bony callus formation, bone marrow from non union site should manifest anomalous activity whatever the origin of the focus sampled. Therefore, the aim of this study was to seek in vitro, using cell cloning techniques, anomalies of bone marrow from non union site. The study characterizes the bone marrow from 35 non union sites, not only with respect to the medullary stroma but also the hematopoietic compartment. In this study, we look for systemic anomalies which could explain susceptibility to non union, we also compare in vitro activity of bone marrow taken from non union with others samples taken from the iliac crest of the same patient. The cell density in the bone marrow in these sites was studied in vitro with material taken from the non union site. Samples were taken by aspiration...Continue Reading

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