Bone marrow mesenchymal stem cell-derived endothelial cells increase capillary density and accelerate angiogenesis in mouse hindlimb ischemia model.

Stem Cell Research & Therapy
Ziping YaoYuan Li

Abstract

Mesenchymal stem cells (MSCs) can improve limb perfusion and increase vessel density in a murine model of hindlimb ischemia. But low engraftment rate of those cells limited their therapeutic effect. Endothelial cells (ECs) play an important role in neovascularization. And MSCs can differentiate into ECs in vitro. The aim of this study is to investigate if EC differentiation of MSCs in vitro before transplantation is effective in improving therapeutic outcomes in the treatment of ischemic disease in a murine ischemia animal model. MSCs were isolated from the bone marrow of EGFP-transgenic mice by density gradient centrifugation. The identity of the MSCs was determined by their cluster of differentiation (CD) marker profile by flow cytometry. Inducing medium containing a few cytokines was applied to induce the MSCs to differentiate into ECs. Endothelial differentiation was quantitatively evaluated using flow cytometry, quantitative real-time PCR (qRT-PCR), immunofluorescence, Matrigel tube formation assay, and Dil-labeled acetylated low-density lipoprotein uptake assay. Mouse hindlimb ischemia model was made by excision of the femoral artery. Uninduced EGFP+ MSCs, induced EGFP+ MSCs, and PBS were intramuscularly injected into the...Continue Reading

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Citations

Jul 20, 2021·Frontiers in Cell and Developmental Biology·Yipeng DuZhiliang Li
Aug 17, 2021·Stem Cell Reviews and Reports·Umberto GalderisiGiovanni Di Bernardo
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Fei SunHongcan Shi
Jan 9, 2022·Nature Reviews. Cardiology·Jonathan Golledge

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Methods Mentioned

BETA
amputation
surgical
flow cytometry
PCR
confocal microscopy
fluorescence microscopy

Software Mentioned

Image
Pro Plus

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