Bone Marrow Mesenchymal Stem Cell Transplantation Increases GAP-43 Expression via ERK1/2 and PI3K/Akt Pathways in Intracerebral Hemorrhage

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Jian-Zhong CuiJun-Ling Gao

Abstract

Intracerebral hemorrhage (ICH) occurs in hypertensive patients and results in high rates of mortality and disability. This study determined whether bone marrow mesenchymal stem cell (BMSC) transplantation affects axonal regeneration and examined the underlying mechanisms after the administration of PD98059 (p-ERK1/2 inhibitor) or/ and LY294002 (PI3K inhibitor). The hypothesis that was intended to be tested was that BMSC transplantation regulates the expression of growth-associated protein-43 (GAP-43) via the ERK1/2 and PI3K/Akt signaling pathways. Seventy-five male rats (250-280 g) were subjected to intracerebral blood injection and then randomly received a vehicle, BMSCs, PD98059 or LY294002 treatment. Neurological deficits were evaluated prior to injury and at 1, 3 and 7 days post-injury. The expression of GAP-43, Akt, p-Akt, ERK1/2, and p-ERK1/2 proteins was measured by western blot analysis. BMSC transplantation attenuated neurological deficits 3-7 days post-ICH. The expression of GAP-43 was increased 3 days following BMSC transplantation. However, this increase was inhibited by either PD98059 or LY294002 treatment. Treatment with both PD98059 and LY294002 was more effective than was treatment with an individual compound. B...Continue Reading

Citations

Jun 7, 2018·Cell Transplantation·Liansheng GaoGao Chen
Mar 29, 2020·International Journal of Molecular Sciences·Eunyoung JungYoon Kyung Choi
May 18, 2020·Molecular Biology Reports·Abel Po-Hao HuangDar-Ming Lai
May 18, 2019·Npj Regenerative Medicine·Marion T TurnbullWilliam D Freeman
Jun 18, 2021·World Journal of Stem Cells·Xi-Min HuKun Xiong
Aug 4, 2021·Neurocritical Care·Kathryn N KearnsDale Ding

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