Abstract
Intracerebral hemorrhage (ICH) occurs in hypertensive patients and results in high rates of mortality and disability. This study determined whether bone marrow mesenchymal stem cell (BMSC) transplantation affects axonal regeneration and examined the underlying mechanisms after the administration of PD98059 (p-ERK1/2 inhibitor) or/ and LY294002 (PI3K inhibitor). The hypothesis that was intended to be tested was that BMSC transplantation regulates the expression of growth-associated protein-43 (GAP-43) via the ERK1/2 and PI3K/Akt signaling pathways. Seventy-five male rats (250-280 g) were subjected to intracerebral blood injection and then randomly received a vehicle, BMSCs, PD98059 or LY294002 treatment. Neurological deficits were evaluated prior to injury and at 1, 3 and 7 days post-injury. The expression of GAP-43, Akt, p-Akt, ERK1/2, and p-ERK1/2 proteins was measured by western blot analysis. BMSC transplantation attenuated neurological deficits 3-7 days post-ICH. The expression of GAP-43 was increased 3 days following BMSC transplantation. However, this increase was inhibited by either PD98059 or LY294002 treatment. Treatment with both PD98059 and LY294002 was more effective than was treatment with an individual compound. B...Continue Reading
Citations
Jun 7, 2018·Cell Transplantation·Liansheng GaoGao Chen
Dec 5, 2018·Cell Transplantation·Weijian FanGang Chen
Mar 29, 2020·International Journal of Molecular Sciences·Eunyoung JungYoon Kyung Choi
May 18, 2020·Molecular Biology Reports·Abel Po-Hao HuangDar-Ming Lai
Jul 2, 2020·Journal of Molecular Neuroscience : MN·Tingting HuangJiajun Xu
Jun 14, 2018·Frontiers in Molecular Neuroscience·Weilin XuJianmin Zhang
May 18, 2019·Npj Regenerative Medicine·Marion T TurnbullWilliam D Freeman
Oct 5, 2018·Frontiers in Neuroscience·Weilin XuJun Yu
Jul 12, 2019·Frontiers in Neuroscience·Yuanzhi LiChuanzhi Duan
Mar 28, 2018·BioMed Research International·Lei ZhangShijie Fu
Jun 9, 2020·Journal of Exercise Rehabilitation·Ji-Eun KimTae-Beom Seo
Nov 17, 2020·Frontiers in Neuroscience·Heyu ZhangZhiyi He
Mar 18, 2021·Human & Experimental Toxicology·F LiangX Zhu
May 1, 2021·Frontiers in Aging Neuroscience·Jing LiHaitao Sun
Jun 29, 2021·Journal of Cellular and Molecular Medicine·Jun ZengZhichong Xie
Jun 18, 2021·World Journal of Stem Cells·Xi-Min HuKun Xiong
Aug 4, 2021·Neurocritical Care·Kathryn N KearnsDale Ding