Bone marrow mesenchymal stem cells combined with ultra-purified alginate gel as a regenerative therapeutic strategy after discectomy for degenerated intervertebral discs

EBioMedicine
Daisuke UkebaNorimasa Iwasaki

Abstract

Because the regenerative ability of intervertebral discs (IVDs) is restricted, defects caused by discectomy may induce insufficient tissue repair leading to further IVD degeneration. An acellular bioresorbable biomaterial based on ultra-purified alginate (UPAL) gel was developed to fill the IVD cavity and prevent IVD degeneration. However, an acellular matrix-based strategy may have limitations, particularly in the elderly population, who exhibit low self-repair capability. Therefore, further translational studies involving product combinations, such as UPAL gel plus bone marrow-derived mesenchymal stem cells (BMSCs), are required to evaluate the regenerative effects of BMSCs embedded in UPAL gel on degenerated IVDs. Rabbit BMSCs and nucleus pulposus cells (NPCs) were co-cultured in a three-dimensional (3D) system in UPAL gel. In addition, rabbit or human BMSCs combined with UPAL gel were implanted into IVDs following partial discectomy in rabbits with degenerated IVDs. Gene expression of NPC markers, growth factors, and extracellular matrix was significantly increased in the NPC and BMSC 3D co-culture compared to that in each 3D mono-culture. In vivo, whereas UPAL gel alone suppressed IVD degeneration as compared to discectomy...Continue Reading

Citations

Jan 23, 2021·The Journal of Bone and Joint Surgery. American Volume·Daisuke UkebaHideki Sudo
Jan 22, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rui-Zhi TangXi-Qiu Liu
Jul 27, 2021·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·Yidian WangXuewen Kang

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BETA
Assay
enzyme-linked immunosorbent assay

Software Mentioned

AnalyzeDirect
Diva
JMP Pro

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