Bone marrow mononuclear cells protect neurons and modulate microglia in cell culture models of ischemic stroke.

Journal of Neuroscience Research
Sushil SharmaSean I Savitz

Abstract

Although several studies have provided evidence for the therapeutic potential of bone marrow-derived mononuclear cells (MNCs) in animal models of stroke, the mechanisms underlying their benefits remain largely unknown. We have determined the neuroprotective potential of MNCs in primary neuronal cultures exposed to various injuries in vitro. Cortical neurons in culture were exposed to oxygen-glucose deprivation, hypoxia, or hydrogen peroxide, and cell death was assayed by MTT, caspase-3 activation or TUNEL labelling at 24 hrs. Cultures were randomized to cotreatment with MNC-derived supernatants or media before injury exposure. In separate experiments, macrophage or microglial cultures were exposed to lipopolypolysacharide (LPS) in the presence and absence of MNC-derived supernatants. Neuronal cultures were then exposed to conditioned media derived from activated macrophages or microglia. Cytokines from the supernantants of MNC cultures exposed to normoxia or hypoxia were also estimated by enzyme-linked immunosorbant assay (ELISA). MNC-derived supernatants attenuated neuronal death induced by OGD, hypoxia, hydrogen peroxide, and conditioned macrophage/microglial media and contain a number of trophic factors, including interleuki...Continue Reading

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Citations

Jun 27, 2012·Stem Cells and Development·Bing YangSean I Savitz
Aug 28, 2012·Experimental & Translational Stroke Medicine·Daniel-Christoph WagnerJohannes Boltze
Apr 27, 2013·International Journal of Nanomedicine·Sushil SharmaManuchair Ebadi
Oct 24, 2012·Vascular Health and Risk Management·Soma BanerjeeJeremy Chataway
Nov 14, 2013·Clinical & Developmental Immunology·Roslyn A Taylor, Lauren H Sansing
Jul 26, 2011·Annals of Neurology·Sean I SavitzJames C Grotta
Jan 30, 2019·Journal of Leukocyte Biology·Ramasatyaveni GeesalaThorsten Maretzky
Mar 20, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helen K Smith, Felicity N E Gavins
Mar 7, 2020·Journal of Clinical Medicine·Katarzyna Jezierska-WozniakWojciech Maksymowicz

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