Abstract
Although several studies have provided evidence for the therapeutic potential of bone marrow-derived mononuclear cells (MNCs) in animal models of stroke, the mechanisms underlying their benefits remain largely unknown. We have determined the neuroprotective potential of MNCs in primary neuronal cultures exposed to various injuries in vitro. Cortical neurons in culture were exposed to oxygen-glucose deprivation, hypoxia, or hydrogen peroxide, and cell death was assayed by MTT, caspase-3 activation or TUNEL labelling at 24 hrs. Cultures were randomized to cotreatment with MNC-derived supernatants or media before injury exposure. In separate experiments, macrophage or microglial cultures were exposed to lipopolypolysacharide (LPS) in the presence and absence of MNC-derived supernatants. Neuronal cultures were then exposed to conditioned media derived from activated macrophages or microglia. Cytokines from the supernantants of MNC cultures exposed to normoxia or hypoxia were also estimated by enzyme-linked immunosorbant assay (ELISA). MNC-derived supernatants attenuated neuronal death induced by OGD, hypoxia, hydrogen peroxide, and conditioned macrophage/microglial media and contain a number of trophic factors, including interleuki...Continue Reading
References
Jan 1, 1997·Molecular and Cellular Neurosciences·F J GottronD W Choi
Sep 3, 1998·Neuroscience Letters·P A SperaF C Barone
Oct 7, 1998·Progress in Neurobiology·G StollM Schroeter
Mar 31, 1999·Journal of Neurochemistry·M SawadaT Nagatsu
Dec 6, 2001·Neuroscience·K JinD A Greenberg
Jun 19, 2003·The Journal of Clinical Investigation·Yunjuan SunDavid A Greenberg
Apr 6, 2004·Brain Research·Satoshi IihoshiJeffery D Kocsis
Jul 1, 2004·Cell Research·Paul Alexander JonesJohn Sharkey
Jan 31, 2006·Brain Research·Xiurong ZhaoJaroslaw Aronowski
Apr 11, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Masaya TakahashiKimikazu Hamano
Dec 26, 2006·Journal of Neuroimmunology·Qing WangMidori A Yenari
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Andrew H BakerClaudio Napoli
Apr 26, 2007·Annals of Neurology·Xiurong ZhaoJaroslaw Aronowski
Sep 29, 2007·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Runjiang QuMichael Chopp
May 3, 2008·Life Sciences·Izumi YawataAkio Suzumura
Feb 7, 2009·Stroke; a Journal of Cerebral Circulation·Ron KooijmanJacques De Keyser
Apr 17, 2009·Brain Research·Arthur Giraldi-GuimardesRosalia Mendez-Otero
Apr 21, 2009·Lancet Neurology·Zheng Gang Zhang, Michael Chopp
Sep 24, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Miranda BrennemanSean I Savitz
Citations
Sep 1, 2011·Translational Stroke Research·Johannes BoltzeHeinz-Adolf Schoon
Jun 27, 2012·Stem Cells and Development·Bing YangSean I Savitz
Mar 5, 2013·BMC Neuroscience·Jian ChenSheng Tan
Aug 28, 2012·Experimental & Translational Stroke Medicine·Daniel-Christoph WagnerJohannes Boltze
Mar 14, 2013·PloS One·Catherine E DownesPeter J Crack
Sep 17, 2013·PloS One·Norihiko NakanoChizuka Ide
Dec 5, 2013·PloS One·Koji AtakaMineko Fujimiya
Apr 27, 2013·International Journal of Nanomedicine·Sushil SharmaManuchair Ebadi
Oct 24, 2012·Vascular Health and Risk Management·Soma BanerjeeJeremy Chataway
Nov 14, 2013·Clinical & Developmental Immunology·Roslyn A Taylor, Lauren H Sansing
Dec 18, 2013·Neurochemical Research·Marcos Maurício Tosta LealMilena Botelho Pereira Soares
Nov 1, 2016·Molecular Neurobiology·Jianping WangJian Wang
Jul 26, 2011·Annals of Neurology·Sean I SavitzJames C Grotta
Jul 31, 2013·Brain, Behavior, and Immunity·Jianping WangJian Wang
May 21, 2013·Behavioural Brain Research·Chao JiangJian Wang
Dec 9, 2010·Brain Research·Sushil SharmaSean I Savitz
Dec 21, 2014·Brain, Behavior, and Immunity·Jianping WangJian Wang
Aug 25, 2017·Translational Stroke Research·Bing YangSean I Savitz
Jan 30, 2019·Journal of Leukocyte Biology·Ramasatyaveni GeesalaThorsten Maretzky
Mar 20, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helen K Smith, Felicity N E Gavins
Mar 7, 2020·Journal of Clinical Medicine·Katarzyna Jezierska-WozniakWojciech Maksymowicz
Oct 13, 2020·Frontiers in Immunology·Fangxi LiuChuansheng Zhao
Sep 19, 2019·Stem Cells·Farhaan S VahidySean I Savitz
Nov 2, 2016·Stem Cells·Charles S CoxRyan S Kitagawa