DOI: 10.1101/469528Nov 18, 2018Paper

Bone marrow-on-a-chip: Emulating the human bone marrow

BioRxiv : the Preprint Server for Biology
Stefan SieberMark Rosowski

Abstract

Multipotent hematopoietic stem and progenitor cells HPSC reside in specialized stem cell niches within the bone marrow, that provide a suitable microenvironment for lifelong maintenance of the stem cells. Meaningful in vitro models recapitulating the in vivo stem cell niche biology can be employed for both basic research as well as for applied sciences and represent a powerful tool to reduce animal tests in preclinical studies. Recently we published the generation of an in vitro bone marrow niche model, capable of long-term cultivation of HSC based on an organ-on-a-chip platform. This study provides a detailed analysis of the 3D culture system including matrix environment analysis by SEM, transcriptome analysis and system intrinsic differentiation induction. Furthermore, the bone marrow on a chip model can serve to multiply and harvest HSPC, since repeated cell removal not compromised the functionality of the culture system. The prolongation of the culture time to 8 weeks demonstrate the capacity to apply the model in repeated drug testing experiments. The quality of the presented system is emphasized by the differentiation capacity of long-term cultivated HSPC in vitro and in vivo. Transplanted human HSCP migrated actively int...Continue Reading

Related Concepts

Bone Marrow
Cell Culture Techniques
Cell Differentiation Process
Hematopoietic Stem Cells
Laboratory mice
Scanning Electron Microscopy
Stem Cells
Transplantation
Laboratory Culture
Research Study

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