Bone marrow-on-a-chip replicates hematopoietic niche physiology in vitro

Nature Methods
Yu-suke TorisawaDonald E Ingber

Abstract

Current in vitro hematopoiesis models fail to demonstrate the cellular diversity and complex functions of living bone marrow; hence, most translational studies relevant to the hematologic system are conducted in live animals. Here we describe a method for fabricating 'bone marrow-on-a-chip' that permits culture of living marrow with a functional hematopoietic niche in vitro by first engineering new bone in vivo, removing it whole and perfusing it with culture medium in a microfluidic device. The engineered bone marrow (eBM) retains hematopoietic stem and progenitor cells in normal in vivo-like proportions for at least 1 week in culture. eBM models organ-level marrow toxicity responses and protective effects of radiation countermeasure drugs, whereas conventional bone marrow culture methods do not. This biomimetic microdevice offers a new approach for analysis of drug responses and toxicities in bone marrow as well as for study of hematopoiesis and hematologic diseases in vitro.

References

Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M SchwartzS G Emerson
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A H Reddi, C Huggins
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·C L Miller, C J Eaves
Mar 12, 2002·Tissue Engineering·Alexander S KrupnickAlan W Flake
Oct 24, 2003·Nature·L M CalviD T Scadden
Oct 13, 2005·Experimental Hematology·Francis Hérodin, Michel Drouet
Oct 20, 2005·Journal of Bioscience and Bioengineering·Mutsumi Takagi
Feb 26, 2008·Cell·Stuart H Orkin, Leonard I Zon
May 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mark B MeadsWilliam S Dalton
Dec 2, 2008·Clinical Oral Implants Research·Z SchwartzB D Boyan
Mar 3, 2009·Seminars in Radiation Oncology·Joel S Greenberger, Michael Epperly
Mar 26, 2010·Radiation Research·Jacqueline P WilliamsWilliam H McBride
Aug 13, 2010·Nature·Simón Méndez-FerrerPaul S Frenette
Oct 19, 2010·Biomaterials·Nunzia Di MaggioIvan Martin
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xu CaoFrank J Frassica
Sep 3, 2011·Nature Reviews. Molecular Cell Biology·Leo D Wang, Amy J Wagers
Nov 16, 2011·Tissue Engineering. Part C, Methods·Matthew M CookMichael Doran
Apr 4, 2012·Radiation Research·Lynnette H CaryMark H Whitnall
May 5, 2012·Lab on a Chip·Dongeun HuhDonald E Ingber
Nov 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Jungwoo LeeBiju Parekkadan
Feb 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Celeste ScottiIvan Martin

❮ Previous
Next ❯

Citations

Aug 6, 2014·Nature Biotechnology·Sangeeta N Bhatia, Donald E Ingber
Aug 30, 2016·Current Opinion in Chemical Engineering·Esak LeeChristopher S Chen
Sep 2, 2014·Advanced Drug Delivery Reviews·Maureen E Lynch, Claudia Fischbach
Dec 26, 2015·Cancer Letters·Jerome LacombeFrederic Zenhausern
Sep 12, 2015·Thrombosis and Haemostasis·A BalduiniD L Kaplan
Jan 27, 2016·Journal of Hematology & Oncology·Mónica S Ventura FerreiraRebekka K Schneider
Aug 5, 2015·Proceedings of the National Academy of Sciences of the United States of America·Amy WuRobert H Austin
Dec 31, 2015·Tissue Engineering. Part B, Reviews·Holly Wobma, Gordana Vunjak-Novakovic
Oct 21, 2015·Kidney Research and Clinical Practice·Sejoong Kim, Shuichi Takayama
Jan 13, 2015·Advanced Drug Delivery Reviews·Mark Chong Seow Khoon
Dec 18, 2014·Cell Stem Cell·Martin EtzrodtTimm Schroeder
Nov 4, 2015·Advanced Drug Delivery Reviews·Ryohichi Sugimura
Mar 20, 2016·Tissue Engineering. Part C, Methods·Yu-Suke TorisawaDonald Ingber
Feb 20, 2016·Expert Review of Molecular Diagnostics·Bjørn T GjertsenJörn Skavland
Sep 12, 2015·Biotechnology Journal·Ji Sun ChoiBrendan A C Harley
Jul 21, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Lauren E JansenShelly R Peyton
Jul 3, 2015·Biotechnology Advances·Mario RothbauerPeter Ertl
Jun 6, 2015·Biomicrofluidics·Shannon L FaleyLeon M Bellan
Apr 14, 2016·Bioanalysis·Chun-Wei ChiSihong Wang
May 23, 2015·Biomaterials·Boris M HolzapfelJean-Pierre Levesque
Jun 27, 2015·Stem Cells International·Mohammad A Al-DreesStuart T Fraser
Apr 22, 2015·Journal of Materials Science. Materials in Medicine·Simone Bersini, Matteo Moretti
Mar 31, 2015·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Jeehye KimHojae Bae
Mar 21, 2015·Nature Reviews. Drug Discovery·Eric W EschDongeun Huh
Jan 13, 2015·Cellular and Molecular Life Sciences : CMLS·Alessandro MalaraAlessandra Balduini
Jan 27, 2015·Annual Review of Pathology·Kambez H BenamDonald E Ingber
Aug 13, 2015·PloS One·Marguerite M VantangoliKim Boekelheide
Jun 9, 2016·Current Opinion in Biotechnology·Yi Liu, Hang Lu
Jun 7, 2016·Drug Discovery Today·Lambert I J C BergersSusan Gibbs
Sep 2, 2016·Nature Protocols·Ali Khademhosseini, Robert Langer
Oct 28, 2016·Nature Cell Biology·Lara Wahlster, George Q Daley
Jul 9, 2016·Stem Cells Translational Medicine·Kiran BattaValerie Kouskoff
Dec 23, 2016·Tissue Engineering. Part C, Methods·Mohammad HoushmandMahin Nikougoftar Zarif
Feb 18, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Nauman KhalidMitsutoshi Nakajima
Apr 28, 2017·Biomaterials Science·Zhichao HaoJun Wu
Oct 18, 2016·Ophthalmic Plastic and Reconstructive Surgery·Michelle T SunDinesh Selva
May 10, 2017·Proceedings of the National Academy of Sciences of the United States of America·Yu-Ru ShihShyni Varghese
Apr 21, 2017·Current Opinion in Hematology·Shivem B Shah, Ankur Singh
Jun 16, 2017·Lab on a Chip·Boyang Zhang, Milica Radisic
Jun 18, 2017·Chemical Communications : Chem Comm·Rachael M KenneyMatthew R Lockett

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
Baba Yoshinobu
Integrative Biology : Quantitative Biosciences From Nano to Macro
Kyung-Jin JangDonald E Ingber
© 2021 Meta ULC. All rights reserved