Bone marrow stromal cells induce BMP2/4 production in oxygen-glucose-deprived astrocytes, which promotes an astrocytic phenotype in adult subventricular progenitor cells

Journal of Neuroscience Research
Hongqi XinMichael Chopp

Abstract

Bone morphogenetic proteins (BMPs) affect cell proliferation and differentiation. Astrocytes in ischemic brain are highly responsive to bone marrow stromal cell (BMSC) treatment. We investigated the effects of BMSCs on astrocytes cultured under oxygen- and glucose-deprived conditions, which in part simulate in vivo stroke conditions, to test the hypothesis that BMSCs alter astrocytic expression of BMPs which may contribute to neurological functional recovery of stroke. Quantitative real-time RT-PCR showed that the expression of BMP2/4 mRNAs decreased within ischemic astrocytes, In contrast, BMP2/4 mRNA was significantly increased after cocultured with BMSCs. Western blotting also confirmed this increase at the protein level in the medium of ischemic astrocytes after coculture with BMSCs. As a source of neural stem and progenitor cells, cultured subventricular zone (SVZ) neurospheres exposed to medium obtained from ischemic astrocytes cocultured with BMSCs were significantly enriched in cells expressing the astrocytic marker glial fibrillary acidic protein (GFAP), but not at the expense of beta-III-tubulin-positive SVZ neuroblasts. The expression of BMP2/4 subsequently increased the phosphorylation of downstream effector Smad1 a...Continue Reading

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Citations

Apr 11, 2013·Ageing Research Reviews·Lavinia AlberiSwananda Marathe
Dec 19, 2009·Stroke; a Journal of Cerebral Circulation·Jayshree SamantaJohn A Kessler
Feb 23, 2013·Stem Cells Translational Medicine·Irina AizmanCasey C Case
Sep 21, 2013·Journal of Molecular Neuroscience : MN·Huan WanYujun Pan
Mar 15, 2012·Hormones and Behavior·Kelli A DuncanColin J Saldanha
Oct 29, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anna SmirkinJunya Tanaka
Jul 6, 2007·Journal of the Neurological Sciences·Michael ChoppJing Zhang
Mar 28, 2008·Journal of Neurochemistry·Bradley J Walters, Colin J Saldanha
Sep 29, 2007·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Runjiang QuMichael Chopp
Oct 13, 2015·Progress in Neurobiology·Zhongwu Liu, Michael Chopp
Nov 27, 2014·Frontiers in Cellular Neuroscience·Hongqi XinMichael Chopp
Aug 11, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hongqi XinMichael Chopp
May 22, 2009·Journal of Neuropathology and Experimental Neurology·Jill M See, Judith B Grinspan
Feb 7, 2021·Stem Cell Research & Therapy·Yamei ZhangChenchen Xie

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