Bone marrow transplantation in mice leads to a minor population of hepatocytes that can be selectively amplified in vivo

Hepatology : Official Journal of the American Association for the Study of Liver Diseases
Vincent O MalletHélène Gilgenkrantz

Abstract

Cell-based therapy may some day be a therapeutic alternative to liver transplantation. Recent observations indicating that hematopoietic stem cells can differentiate into hepatocytes have opened new therapeutic prospects. However, the clinical relevance of this phenomenon is unknown. We have previously developed a strategy based on the protective effect of Bcl-2 against Fas-mediated apoptosis to selectively amplify a small number of hepatocytes in vivo. We now show that this approach can be used to amplify a minor population of bone marrow-derived hepatocytes. Normal mice were transplanted with unfractionated bone marrow cells from transgenic animals expressing Bcl-2 under the control of a liver-specific promoter. Recipients were then submitted to weekly injections of the anti-Fas antibody, Jo2. Upon sacrifice, the liver of the recipients showed bone marrow-derived clusters of mature hepatocytes expressing Bcl-2, which showed that the hepatocyte progeny of a genetically modified bone marrow can be selectively expanded in vivo. In contrast, no Bcl-2 expression could be detected without the selective pressure of Jo2, suggesting that differentiation of bone marrow cells into mature hepatocytes is very inefficient under physiologic...Continue Reading

References

May 15, 1999·Science·B E PetersenJ P Goff
Dec 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N D TheiseD S Krause
Jun 22, 2000·Human Molecular Genetics·C MitchellH Gilgenkrantz
Jun 28, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·N D TheiseD S Krause
Aug 5, 2000·Nature·M R AlisonN A Wright
Apr 5, 2001·Nature·D OrlicP Anversa
May 5, 2001·Immunity·E LagasseI L Weissman
Jun 8, 2001·The Journal of Clinical Investigation·K A JacksonM A Goodell
Jun 29, 2001·Nature·G FerrariF Mavilio
Jul 6, 2001·Cell·H M BlauJ M Weimann
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E GuidottiH Gilgenkrantz
Aug 16, 2001·Proceedings of the National Academy of Sciences of the United States of America·D OrlicP Anversa

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Citations

Jul 13, 2002·The Journal of Pathology·Stuart ForbesMalcolm Alison
Feb 26, 2004·International Journal of Hematology·Graça Almeida-PoradaEsmail D Zanjani
Jul 5, 2005·Journal of Hepato-biliary-pancreatic Surgery·Kenichi TeramotoShigeki Arii
Jul 5, 2005·Journal of Hepato-biliary-pancreatic Surgery·Takahisa FujikawaBryon E Petersen
Sep 1, 2005·Surgery Today·Yujo KawashitaTakashi Kanematsu
Feb 6, 2004·Blood Cells, Molecules & Diseases·Graça Almeida-Porada, Esmail D Zanjani
Nov 13, 2008·Cytotechnology·Neil D Theise
Sep 27, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Irene Oi-Lin NgSheung-Tat Fan
Aug 7, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xin WangMarkus Grompe
Sep 28, 2004·Journal of Pediatric Gastroenterology and Nutrition·M Najimi, E Sokal
Jan 30, 2004·Journal of Clinical Pathology·M R AlisonN A Wright
Feb 2, 2008·World Journal of Gastroenterology : WJG·Philippe A LysyEtienne M Sokal
Oct 26, 2011·World Journal of Gastroenterology : WJG·Patrizia BurraFrancesco Paolo Russo
Dec 7, 2002·Seminars in Cell & Developmental Biology·Heather A CrosbyAlastair J Strain
Dec 7, 2002·Seminars in Cell & Developmental Biology·Neil D Theise, Diane S Krause
Sep 1, 2012·Translational Stroke Research·Xian Nan TangMidori A Yenari
Jun 5, 2003·Expert Opinion on Biological Therapy·Neil D Theise
Apr 27, 2010·Experimental Hematology·Graça Almeida-PoradaChristopher D Porada
Aug 5, 2008·Journal of Pediatric Surgery·Scott V PerrymanKarl G Sylvester
Jul 1, 2008·Gastroenterology·Diarmaid Dominic Houlihan, Philip Noel Newsome
Apr 25, 2006·Gastroentérologie Clinique Et Biologique·Niaz Kohneh-ShahriOlivier Soubrane
Mar 15, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Delia D'AvolaJorge Quiroga
Oct 21, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Belinda KnightGeorge C Yeoh
Nov 24, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Hélène Gilgenkrantz
May 25, 2005·The British Journal of Surgery·I O L Ng
Jun 17, 2005·Annals of the New York Academy of Sciences·Diane S Krause
Apr 2, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ryan A McTaggart, Sandy Feng
Jul 17, 2004·International Journal of Experimental Pathology·Te-Chao FangRichard Poulsom
Mar 30, 2004·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Marc H DahlkeJohn E J Rasko
Jun 9, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nelson Fausto
May 14, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ping ZhouJan Nolta
Jan 29, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Konrad L StreetzMark A Kay
Nov 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bruno Christ, Sandra Pelz
Apr 21, 2010·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Nagwa ElkhafifGihan El Fandy
Dec 8, 2004·Journal of Hepatology·Patrizia BurraSanjeev Gupta
Jan 18, 2006·Transplant Immunology·K J AllenR Williamson
Oct 18, 2005·Journal of Hepatology·Marc H DahlkePatrick Bertolino

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