Bone mineral and glycosaminoglycans in newborn and mature rabbits

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
M D Grynpas, G K Hunter

Abstract

The relation between bone mineralization and glycosaminoglycans (GAG) was investigated in newborn and mature rabbit diaphyseal bone. Using the density fractionation technique, the bone was separated into fractions of increasing density from 1.4 to 2.3 grams/ml. Each fraction was analyzed by X-ray diffraction to determine the average crystal size. The GAG content of each fraction and of the unfractionated bone was determined by direct extraction and on a few fractions by sequential extraction in guanidine hydrochloride and guanidine/EDTA. There was a decrease in the GAG content with animal age and with increasing fraction density in the newborn rabbit. In one overlapping fraction (2.0-2.1 grams/ml), the GAG content was twice as high in the newborn as in the mature animal. Finally, the crystal size substantially increased from newborn to mature rabbits. Therefore, calcification and maturation of bone is associated with a decrease in the proteoglycan content of the organic matrix.

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Citations

Aug 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M D GrynpasM J Kessler
Mar 22, 2003·Journal of Structural Biology·I ZizakP Fratzl
Feb 7, 2008·The Journal of Biological Chemistry·Jan C-C HuJames P Simmer
Mar 1, 1992·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M D GrynpasJ M Very
Mar 21, 2009·Journal of Biomedical Materials Research. Part a·Andrew K LynnWilliam Bonfield
Mar 21, 2009·Journal of Biomedical Materials Research. Part a·Brendan A HarleyLorna J Gibson
Jan 1, 1993·Calcified Tissue International·M Grynpas
Sep 18, 2009·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M Saito, K Marumo
Dec 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B D Boyan, Z Schwartz
Feb 24, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K HoshiH Ozawa
Jun 16, 2001·Journal of Biomedical Materials Research·J Lovekamp, N Vyavahare
Mar 23, 2006·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M SaitoT Tanaka
Jul 1, 1991·Calcified Tissue International·M D GrynpasK P Pritzker
Jul 1, 2004·Journal of Biomedical Materials Research. Part a·Rachit OhriCecilia M Giachelli
Oct 1, 1994·Calcified Tissue International·M D GrynpasB P Mertz
Jan 1, 1993·Journal of Biomedical Materials Research·J G SkedrosB Constantz

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