Bone Morphogenetic Protein 2 Coordinates Early Tooth Mineralization

Journal of Dental Research
Z MalikD Graf

Abstract

Formation of highly organized dental hard tissues is a complex process involving sequential and ordered deposition of an extracellular scaffold, followed by its mineralization. Odontoblast and ameloblast differentiation involves reciprocal and sequential epithelial-mesenchymal interactions. Similar to early tooth development, various Bmps are expressed during this process, although their functions have not been explored in detail. Here, we investigated the role of odontoblast-derived Bmp2 for tooth mineralization using Bmp2 conditional knockout mice. In developing molars, Bmp2LacZ reporter mice revealed restricted expression of Bmp2 in early polarized and functional odontoblasts while it was not expressed in mature odontoblasts. Loss of Bmp2 in neural crest cells, which includes all dental mesenchyme, caused a delay in dentin and enamel deposition. Immunohistochemistry for nestin and dentin sialoprotein (Dsp) revealed polarization defects in odontoblasts, indicative of a role for Bmp2 in odontoblast organization. Surprisingly, pSmad1/5/8, an indicator of Bmp signaling, was predominantly reduced in ameloblasts, with reduced expression of amelogenin ( Amlx), ameloblastin ( Ambn), and matrix metalloproteinase ( Mmp20). Quantitativ...Continue Reading

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Citations

Jun 10, 2020·International Journal of Molecular Sciences·Juliana BaranovaEdda Tobiasch
Oct 2, 2019·Cells·Yu JiChengji J Zhou
Jun 3, 2021·Materials·Mihaela OlaruGabriela Calin
Jul 13, 2021·Frontiers in Cell and Developmental Biology·Pranidhi BaddamDaniel Graf

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BETA
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scanning electron microscopy

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Excel
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