Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2.

The Journal of Biological Chemistry
Youn-Jeong KimH M Ryoo

Abstract

Alkaline phosphatase (ALP) is a widely accepted bone marker. Its expression is stimulated by bone morphogenetic protein (BMP)-2 treatment, the activation of BMP receptors and R-Smads, and the expression of Dlx5 and Runx2. However, how BMP-2 induces ALP expression is not clearly understood. We dissected the murine ALP promoter and found within it a Dlx5-binding cis-acting element by electrophoretic mobility shift assays and site-directed mutagenesis of the element. Dlx5 and the product of its target gene, Runx2, stimulated ALP promoter activity in an additive manner. However, because Dlx5 continued to stimulate ALP expression in Runx2(-/-) cells, the ALP stimulatory activity of Dlx5 is independent of Runx2. We also found that overexpression of Msx2 suppressed the mRNA level and enzyme activity of ALP that were induced by BMP-2 stimulation, and suppressed the Dlx5-stimulated ALP promoter activity by competing with Dlx5 for the cis-acting element in the ALP promoter. Moreover, Msx2 levels are constitutively high in C2C12 myogenic cells but decrease over time after BMP-2 treatment. This may explain why BMP-2 treatment of these cells results in immediate Dlx5 expression yet ALP expression commences only 1-2 days later. In other word...Continue Reading

References

Oct 1, 1995·Trends in Genetics : TIG·D Davidson
Apr 6, 1995·Biochemical and Biophysical Research Communications·G L SemenzaR Kundu
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·H M HoffmannJ L Stein
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·D W StockK M Weiss
Apr 1, 1996·Mechanisms of Development·K M CatronC Abate-Shen
May 1, 1997·Molecular and Cellular Biology·H ZhangC Abate-Shen
Aug 26, 1997·Biochemistry·E P NewberryD A Towler
Apr 29, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M BidderD A Towler
Mar 6, 1999·The Journal of Biological Chemistry·H HaradaM Nakatsuka
Apr 15, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·I SembaH C Slavkin
Apr 25, 2000·Gene·A J Bendall, C Abate-Shen
Oct 23, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·K TakahashiL Shum
Oct 31, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K ShirakabeE Nishida
Dec 17, 2002·Developmental Biology·Deborah Ferrari, Robert A Kosher
Aug 20, 2003·The Journal of Biological Chemistry·Su-Li ChengDwight A Towler
Sep 10, 2003·Biochemical and Biophysical Research Communications·Mi-Hye LeeHyun-Mo Ryoo
Jun 4, 2004·The Journal of Biological Chemistry·Fumitaka IchidaToshiyuki Yoneda
Jan 1, 1923·The Biochemical Journal·R Robison

❮ Previous
Next ❯

Citations

Nov 12, 2005·Histochemistry and Cell Biology·Kenta KishimotoSohei Kitazawa
Feb 14, 2012·Cell and Tissue Research·Karen RuschkePetra Knaus
May 13, 2008·Journal of Bone and Mineral Metabolism·Riko NishimuraToshiyuki Yoneda
Aug 26, 2011·Cytotechnology·Laddawun Sununliganon, Weerachai Singhatanadgit
May 5, 2007·Journal of Biomedical Science·Shuang YangTianhui Zhu
Jul 21, 2009·The Journal of Biological Chemistry·Young-Dan ChoHyun-Mo Ryoo
Jun 6, 2009·The Journal of Biological Chemistry·Jinfei Xu, Joseph R Testa
May 14, 2010·The Journal of Biological Chemistry·Gin-Ah SongHyun-Mo Ryoo
Jun 16, 2010·The Journal of Biological Chemistry·Ling LingSimon M Cool
Sep 21, 2010·The Journal of Biological Chemistry·Ji Hae JunJeong-Hwa Baek
Aug 16, 2008·The Journal of Biological Chemistry·Takuma MatsubaraToshiyuki Yoneda
Mar 12, 2010·Tissue Engineering. Part C, Methods·Emma L SmithAlastair J Sloan
Jan 14, 2011·Human Molecular Genetics·Samin A SajanMichael Lovett
Sep 25, 2008·The Journal of Craniofacial Surgery·Darren M SmithJoseph E Losee
Dec 10, 2009·Molecular and Cellular Biology·Aditi MukherjeePeter Rotwein
Jan 1, 2008·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Jess Frith, Paul Genever
Oct 20, 2009·BMC Cancer·Stefan NagelRoderick Af Macleod
Dec 25, 2008·Journal of Oral Science·Yasunori HasegawaKoichi Ito
May 5, 2010·Experimental & Molecular Medicine·Hye-Lim LeeJeong-Hwa Baek
Aug 10, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Wei LeiBibhash C Paria
Dec 3, 2014·Journal of Cranio-maxillo-facial Surgery : Official Publication of the European Association for Cranio-Maxillo-Facial Surgery·Yahya AçilMatthias Gierloff
Sep 5, 2014·Journal of Cellular Physiology·Won-Joon YoonHyun-Mo Ryoo
Feb 14, 2013·Journal of Endodontics·Kuan-Liang ChenKuo Yuan
Jan 12, 2016·International Journal of Biological Macromolecules·S VimalrajN Selvamurugan
Nov 11, 2009·Cytokine & Growth Factor Reviews·Slobodan Vukicevic, Lovorka Grgurevic
Mar 27, 2010·Journal of Neurodevelopmental Disorders·Mario MairaStefano Stifani
Apr 28, 2009·Biochemical and Biophysical Research Communications·Jae Hee SeoKwang-Youl Lee
Mar 24, 2009·European Journal of Cell Biology·F Philipp SeibMartin Bornhäuser
Jul 16, 2008·European Journal of Cell Biology·Anita MuragliaGiorgio Corte
Aug 19, 2015·Nanomedicine : Nanotechnology, Biology, and Medicine·Ruizhong ZhangKenneth K Y Wong
Aug 25, 2015·Experimental Cell Research·Takahiro Koyama, Kazuo Kamemura

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.