Bone morphogenetic protein-2 stimulates chondrogenic expression in human nasal chondrocytes expanded in vitro

Growth Factors
Aurélie HautierFrédéric Mallein-Gerin

Abstract

Articular cartilage contains an extracellular matrix with characteristic macromolecules such as type II collagen. Because this tissue is avascular and mature chondrocytes do not proliferate, cartilage lesions have a limited capacity for healing after trauma. Autologous chondrocyte implantation (ACI) is widely used for the treatment of patients with focal damage to articular cartilage. However, this method faces a major issue: dedifferentiation of chondrocytes occurs during the long-term culture necessary for mass cell production. The aim of this study was to determine if the step of cell amplification required for ACI could benefit from the use of bone morphogenetic protein (BMP)-2, a potent regulator of chondrogenic expression. Chondrocytes were isolated from human nasal cartilage, a hyaline cartilage like articular cartilage and were serially cultured in monolayers. After one, two or three passages, BMP-2 was used to evaluate the chondrogenic potential of the dedifferentiated chondrocytes, at the gene and protein level. We found that BMP-2 can reactivate the program of chondrogenic expression in dedifferentiated chondrocytes. To gain insight into the molecular mechanisms involved in the responsiveness of chondrocytes to BMP-2...Continue Reading

References

Jul 1, 1989·Physiological Reviews·P V HauschkaC M Gundberg
Feb 1, 1985·Developmental Biology·T M Schmid, T F Linsenmayer
Jun 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·V C LuiK S Cheah
Jan 1, 1995·International Review of Cytology·R CanceddaP Castagnola
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Feb 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L J SandellR A Reife
Nov 1, 1996·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L Z SailorE A Morris
Apr 1, 1997·Molecular and Cellular Biology·V LefebvreB de Crombrugghe
Jun 1, 1997·Nature Genetics·D M BellK S Cheah
Nov 14, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A OganesianL J Sandell
May 13, 1999·Nature Genetics·W BiB de Crombrugghe
Sep 22, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B K ZehentnerH Burtscher
Jan 26, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M C StewartJ N Macleod
May 20, 2000·Clinical Orthopaedics and Related Research·L PetersonA Lindahl
Apr 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H D NahT Kirsch
Dec 23, 2003·Cellular Signalling·Anja NoheNils O Petersen
Feb 8, 2005·Biochemical and Biophysical Research Communications·Mei Wan, Xu Cao
Jun 29, 2005·Biochemical and Biophysical Research Communications·Gallic BeauchefPhilippe Galéra
Sep 8, 2006·Journal of Biomedical Materials Research. Part a·C VinatierJ Guicheux
Sep 20, 2007·European Journal of Mass Spectrometry·Z OuyangR G Cooks
Mar 1, 1990·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Frédéric Mallein-Gerin

❮ Previous
Next ❯

Citations

Sep 7, 2013·Cell Communication and Signaling : CCS·Jinsoo SongEun-Jung Jin
Dec 29, 2015·Osteoarthritis and Cartilage·Jérôme E LafontFrédéric Mallein-Gerin
Jun 24, 2011·Injury·Anne-Marie Freyria, Frédéric Mallein-Gerin
Sep 24, 2013·Matrix Biology : Journal of the International Society for Matrix Biology·Elisabeth Aubert-FoucherFrédéric Mallein-Gerin
Nov 11, 2016·Tissue Engineering. Part C, Methods·Pedram AkbariWalid A Farhat
Oct 28, 2008·Tissue Engineering. Part a·Anne-Marie FreyriaFrédéric Mallein-Gerin
Jun 11, 2011·Biotechnology Letters·Emeline PerrierAnne-Marie Freyria
Jun 20, 2017·International Journal of Molecular Medicine·Bin ZhouJide Shi
Sep 6, 2020·The Journal of Craniofacial Surgery·Kyu Nam KimJong Woo Choi
Mar 12, 2021·The Journal of Craniofacial Surgery·Kyu Nam KimJong Woo Choi
Feb 5, 2010·European Journal of Cell Biology·Jérôme GouttenoireFrédéric Mallein-Gerin

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.