Bone morphogenetic protein-6 production in human osteoblastic cell lines. Selective regulation by estrogen

The Journal of Clinical Investigation
D J RickardB L Riggs

Abstract

Bone morphogenetic proteins (BMPs) induce differentiation of osteoblast and chondroblast lineage cells from uncommitted mesenchymal precursors. Because estrogen has potent osteochondrogenic actions, we investigated its effect on BMP production in two estrogen-responsive, human immortalized cell lines (hFOB/ER3 and hFOB/ER9) that display the mature osteoblast phenotype. These cell lines were produced by stable transfection of the estrogen receptor (ER) gene into immortalized fetal osteoblasts at low ( approximately 800 ER/ nucleus) and at high ( approximately 3, 900 ER/nucleus) levels, respectively. As assessed by reverse transcriptase PCR, treatment with 17beta-estradiol (10(-)10 - 10(-)7 M) increased steady-state levels of BMP-6 mRNA dose dependently by twofold in the hFOB/ER3 cells and by over threefold in the hFOB/ER9 cells. Messenger RNA levels for transforming growth factors-beta1 and -beta2 and BMPs-1 through -5 and -7 levels were unchanged. The results were confirmed by sequence determination of the PCR product and by Northern blot analysis for total RNA. 17beta-estradiol increased BMP-6 protein production sixfold by Western analysis. Cotreatment with antiestrogens (ICI 182,780 or 4-hydroxytamoxifen) antagonized the effe...Continue Reading

References

Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M J OurslerT C Spelsberg
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A J CelesteJ M Wozney
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E A WangP LaPan
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S VukicevicA H Reddi
Sep 1, 1988·Molecular Endocrinology·A Weisz, F Bresciani
Nov 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·V RosenJ M Wozney
Feb 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S A HarrisT C Spelsberg
Mar 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D E Carey, X Liu
Feb 2, 1995·The New England Journal of Medicine·S C Manolagas, R L Jilka
Dec 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·A S Levenson, V C Jordan
Oct 20, 1994·The New England Journal of Medicine·E P SmithK S Korach
Dec 1, 1993·Endocrine Reviews·M SchuchardT C Spelsberg
Dec 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A H Reddi, N S Cunningham
Jan 1, 1994·Trends in Genetics : TIG·D M Kingsley
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·M J OurslerT Spelsberg
Jan 1, 1993·Breast Cancer Research and Treatment·M G Parker
Aug 1, 1993·Calcified Tissue International·H Ben-HurA Ornoy
Mar 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R T TurnerG K Wakley
Jan 19, 1996·Science·E KesslerD S Greenspan

❮ Previous
Next ❯

Citations

Sep 21, 2011·Archives of Gynecology and Obstetrics·Farfaras AthanasiosKassanos Demetrios
Feb 6, 2004·Toxicology and Applied Pharmacology·Toshiko Yamada-OkabeHisafumi Yamada-Okabe
Jul 9, 2003·European Journal of Biochemistry·Toshiko Yamada-OkabeHisafumi Yamada-Okabe
Dec 29, 2007·BMC Musculoskeletal Disorders·Iwona KochanowskaKazimierz Ostrowski
Sep 27, 2008·Molecular Endocrinology·Susan A KrumMyles Brown
Jul 5, 2003·Biological & Pharmaceutical Bulletin·Leticia Gomez RaoXizhong Zhang
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M SatoN Yasui
Mar 30, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H TamadaS Kitazawa
Mar 29, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·David J RickardThomas C Spelsberg
May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Andrea Plant, Jonathan H Tobias
Mar 6, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rama GarimellaH Clarke Anderson
May 23, 2012·PloS One·Prem Swaroop YadavAmitabha Bandyopadhyay
Apr 13, 2006·Archives of Histology and Cytology·Snjezana MartinovicSlobodan Vukicevic
Nov 11, 2009·Cytokine & Growth Factor Reviews·Slobodan Vukicevic, Lovorka Grgurevic
Jan 27, 2011·Journal of Cellular Biochemistry·Susan A Krum
Oct 12, 2013·Journal of Cellular Physiology·Hwa Young ChoChan Soo Shin
Jul 13, 2002·American Journal of Obstetrics and Gynecology·Michael E CarleyThomas C Spelsberg
Aug 6, 2010·Molecular and Cellular Endocrinology·Ulrika IslanderHans Carlsten
Jun 29, 2001·Japanese Journal of Cancer Research : Gann·T KusumiH Kudo
Mar 26, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Delia B OngJonathan H Tobias
Feb 23, 2008·Journal of Tissue Engineering and Regenerative Medicine·P C BessaR L Reis
Dec 1, 2005·Journal of Cellular Biochemistry·Michael S FriedmanKurt D Hankenson
Jun 20, 2002·Endocrinology·Tetsuya YamamotoTadashi Matsuda
Jan 1, 2007·Expert Review of Endocrinology & Metabolism·Regina EbertFranz Jakob
Jul 21, 2007·Endocrine Reviews·Rainer H Straub
Apr 18, 2003·Endocrine Reviews·Ernesto CanalisElisabetta Gazzerro
Jun 24, 1999·Molecular Endocrinology·T C SpelsbergS Khosla
Jan 12, 2001·Physiological Reviews·J E Compston
Mar 30, 2017·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·S GalliR Jimbo
Jun 26, 2018·Frontiers in Bioengineering and Biotechnology·Roland M Klar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Andrea Plant, Jonathan H Tobias
Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA
L G RaoT M Murray
© 2021 Meta ULC. All rights reserved