Bone Regeneration Using Adipose-Derived Stem Cells in Injectable Thermo-Gelling Hydrogel Scaffold Containing Platelet-Rich Plasma and Biphasic Calcium Phosphate

International Journal of Molecular Sciences
Han-Tsung LiaoJyh-Ping Chen

Abstract

For bone regeneration, a biocompatible thermo-gelling hydrogel, hyaluronic acid-g-chitosan-g-poly(N-isopropylacrylamide) (HA-CPN) was used as a three-dimensional organic gel matrix for entrapping rabbit adipose-derived stem cells (rASCs). Biphasic calcium phosphate (BCP) ceramic microparticles were embedded within the gel matrix as a mineralized bone matrix, which was further fortified with platelet-rich plasma (PRP) with osteo-inductive properties. In vitro culture of rASCs in HA-CPN and HA-CPN/PRP/BCP was compared for cell proliferation and osteogenic differentiation. Overall, HA-CPN/PRP/BCP was a better injectable cell carrier for osteogenesis of rASCs with increased cell proliferation rate and alkaline phosphatase activity, enhanced calcium deposition and mineralization of extracellular matrix, and up-regulated expression of genetic markers of osteogenesis. By implanting HA-CPN/PRP/BCP/rASCs constructs in rabbit critical size calvarial bone defects, new bone formation at the defect site was successfully demonstrated from computed tomography, and histological and immunohistochemical analysis. Taken together, by combining PRP and BCP as the osteo-inductive and osteo-conductive factor with HA-CPN, we successfully demonstrated ...Continue Reading

References

Aug 1, 1989·Calcified Tissue International·G DaculsiD Mitre
Aug 1, 1988·Advances in Dental Research·R Z LeGeros
Apr 30, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L A SolchagaA I Caplan
Sep 17, 1999·Journal of Biomedical Materials Research·K G MarraL E Weiss
Mar 14, 2002·Biochemical and Biophysical Research Communications·Felicity R A J Rose, Richard O C Oreffo
Feb 1, 2003·Differentiation; Research in Biological Diversity·Nicole I zur NiedenHans J Ahr
Feb 26, 2004·The American Journal of Pathology·H Clarke AndersonRama Dhamyamraju
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Apr 16, 2004·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Robert E Marx
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xiaojun YuCato T Laurencin
Oct 7, 2004·Macromolecular Bioscience·António J SalgadoRui L Reis
Dec 26, 2006·Journal of Biomedical Materials Research. Part a·Aylin Sendemir-Urkmez, Russell D Jamison
May 18, 2007·Advanced Drug Delivery Reviews·James D KretlowAntonios G Mikos
Jun 8, 2007·Journal of Materials Science. Materials in Medicine·Huaping TanChangyou Gao
Dec 21, 2007·Biomaterials·Borhane H FellahPierre Layrolle
Nov 14, 2008·Chemical Reviews·Racquel Zapanta LeGeros
Sep 30, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Juan ShenShibo Gao
Oct 1, 2010·Advanced Materials·Brandon V SlaughterNicholas A Peppas
Feb 12, 2011·Journal of Tissue Engineering and Regenerative Medicine·Juan Manuel FernandezAna M Cortizo
Feb 18, 2012·The Journal of Craniofacial Surgery·Gary F Rogers, Arin K Greene
Sep 6, 2013·Tissue Engineering. Part B, Reviews·Han-Tsung LiaoJ Peter Rubin
Jul 8, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Sheeny Lan Levengood, Miqin Zhang
Nov 13, 2015·International Journal of Molecular Sciences·Kwang-Won ParkHae-Ryong Song
Mar 24, 2017·Aesthetic Surgery Journal·Robert P GerschIvona Percec
Apr 2, 2016·Materials·Gianluca TozziMarta Roldo
Mar 20, 2018·Scientific Reports·Anny Waloski RobertMarco Augusto Stimamiglio
Mar 28, 2018·Experimental and Therapeutic Medicine·Hao LiuGuang-Hua Yuan

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Citations

Nov 8, 2018·International Journal of Molecular Sciences·Chiara ArgentatiSabata Martino
Nov 30, 2019·Stem Cells International·Gabriele StortiValerio Cervelli
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·K LavanyaN Selvamurugan
Mar 19, 2021·Regenerative Biomaterials·Manyu ChenXingdong Zhang
Mar 23, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Shujun WangBaoan Chen
Jun 5, 2019·Current Medicinal Chemistry·Fabián Ávila-Salas, Esteban F Durán-Lara
Oct 20, 2020·Journal of Oral Biology and Craniofacial Research·Divya MehrotraR K Singh

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Methods Mentioned

BETA
xenografts
X-Ray
ELISA
enzyme-linked immunosorbent assay
PCR

Software Mentioned

Oligo

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