Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size

PloS One
Takamitsu KogaIzumi Asahina

Abstract

This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance betwee...Continue Reading

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Citations

Apr 14, 2019·Journal of Tissue Engineering and Regenerative Medicine·Jie LiJinlin Song
Jun 19, 2019·Journal of Tissue Engineering·Arooj MunirSalwa Suliman
Jul 11, 2020·The Journal of Craniofacial Surgery·Ufuk TaşdemirYavuz Dodurga
Jul 22, 2017·Journal of Applied Biomaterials & Functional Materials·Nina BonoGabriele Candiani
Nov 3, 2020·The Journal of Craniofacial Surgery·Ufuk TaşdemirYavuz Dodurga
Nov 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sara BernardiSerena Bianchi
Apr 4, 2021·International Journal of Environmental Research and Public Health·Michael Korsch, Marco Peichl
May 1, 2021·Tissue Engineering and Regenerative Medicine·Shuxin ZhangEui-Seok Lee
Jun 16, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·Hefeng YangSong Li
Jan 7, 2021·The Journal of Craniofacial Surgery·Ezgi Yüceer-ÇetinerMehmet Emin Önger
Aug 11, 2021·Tissue Engineering. Part C, Methods·Jeong-Kui KuIn-Woong Um
Aug 28, 2021·International Journal of Molecular Sciences·Serena BianchiSara Bernardi

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Methods Mentioned

BETA
xenografts
scanning electron microscopy

Software Mentioned

ARRIVE
TRI
3D
- BON
NIH Imaging Image - J

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