Bone regeneration with adipose derived stem cells in a rabbit model

Journal of Biomedical Research
Michele MaglioneLorenzo Bevilacqua

Abstract

It has been shown that stem cells are able to calcify both in vitro and in vivo once implanted under the skin, if conveniently differentiated. Nowadays, however, a study on their efficiency in osseous regeneration does not exist in scientific literature and this very task is the real aim of the present experimentation. Five different defects of 6 mm in diameter and 2 mm in depth were created in the calvaria of 8 white New Zealand rabbits. Four defects were regenerated using 2 different conveniently modified scaffolds (Bio-Oss® Block and Bio-Oss Collagen®, Geistlich), with and without the aid of stem cells. After the insertion, the part was covered with a collagen membrane fixed by 5 modified titan pins (Altapin®). The defect in the front was left empty on purpose as an internal control to each animal. Two animals were sacrificed respectively after 2, 4, 6, 10 weeks. The samples were evaluated with micro-CT and histological analysis. Micro-CT analysis revealed that the quantity of new bone for samples with Bio-Oss® Block and stem cells was higher than for samples with Bio-Oss® Block alone. Histological analysis showed that regeneration occurred in an optimal way in every sample treated with scaffolds. The findings indicated that...Continue Reading

Citations

Feb 27, 2020·Tissue Engineering. Part B, Reviews·Mirasbek KuterbekovCatherine Picart
Dec 16, 2018·International Journal of Molecular Sciences·Susanne JungSonja Sielker
Jan 10, 2021·Calcified Tissue International·Dilcele Silva Moreira DziedzicKatherine Athayde Teixeira de Carvalho

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BETA
sedation
X-ray

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PORE3D
OsiriX
IDL
Image ProPlus
Cobra Exxim
GEHC MicroView

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