Bone-related gene profiles in developing calvaria

Gene
Je-Yoel ChoHyun-Mo Ryoo

Abstract

Generating a comprehensive understanding of osteogenesis-related gene profiles is very important in the development of new treatments for osteopenic conditions. Developing calvaria undergoes a typical intramembranous bone-forming process. To identify genes associated with osteoblast differentiation, we isolated total RNAs from parietal bones, that represent active osteoblasts, and sutural mesenchyme, that represents osteoprogenitor cells, and comprehensively analyzed their gene expression profiles using an oligo-based Affymetrix microarray chip containing 22,690 probes. About 2100 genes with "Present" calls had more than 2-fold higher expression in bone compared to sutures while 73 of these genes had more than 8-fold expression. Some of these genes are already known to be bone-related biomarkers: VitD receptor, bone sialoprotein, osteocalcin, osteopontin, MMP13, etc. Eight genes were selected and subjected to confirmation by quantitative real-time RT-PCR analyses. All the genes tested showed higher expression in bones, ranging from 5- to 140-fold. Several of these genes are ESTs while others are already known but their functions in osteogenesis were not previously known. Most genes of the BMP and FGF families probed in the Gene...Continue Reading

References

Jan 20, 1994·Nature·N A MorrisonJ A Eisman
Aug 1, 1996·Nature·P DucyG Karsenty
Jan 1, 1997·Nature Genetics·V el GhouzziJ Bonaventure
Jun 25, 1999·Mechanisms of Development·J XuD M Ornitz
Sep 1, 1999·European Journal of Oral Sciences·D P RiceI Thesleff
Feb 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SenD A Carson
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·K HofmannW Stoffel
Jul 25, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M J Beanan, T D Sargent
Nov 7, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P KettunenI Thesleff
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L A Opperman
May 9, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M H ParkH M Ryoo
May 18, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S M Orestes-CardosoA Berdal
Oct 31, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K ShirakabeE Nishida
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·T D Schmittgen
Aug 24, 2002·Science·Michael J DepewJohn L R Rubenstein
Sep 11, 2002·Biochemical and Biophysical Research Communications·Xiaoyun XingJueren Lou
Oct 9, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jian Q FenMary MacDougall
Oct 10, 2002·American Journal of Physiology. Cell Physiology·Stéphanie G DuboisDaniel Lajeunesse
Nov 16, 2002·Nature Genetics·Mondira KunduP Paul Liu
Dec 10, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Diana S de JongWilma T Steegenga
Feb 5, 2003·The Journal of Biological Chemistry·Norma MarchesiniYusuf A Hannun
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michael A IgnelziAndrew T Young
Apr 11, 2003·Nature·Stephen M WarrenMichael T Longaker
Jun 20, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hyun-Jung KimHyun-Mo Ryoo
Jul 8, 2003·Genome Biology·Joseph P Stains, Roberto Civitelli
Aug 16, 2003·Bone·D P C RiceI Thesleff
Sep 10, 2003·Biochemical and Biophysical Research Communications·Mi-Hye LeeHyun-Mo Ryoo

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Citations

Jul 21, 2009·The Journal of Biological Chemistry·Young-Dan ChoHyun-Mo Ryoo
May 4, 2011·The Journal of Biological Chemistry·Christopher J ClarkeYusuf A Hannun
Apr 2, 2013·Plastic and Reconstructive Surgery·Christopher D HermannBarbara D Boyan
Sep 29, 2011·Yi chuan = Hereditas·Yan-Yan QianDuan Ma
Jan 26, 2007·Clinical Genetics·G R HandriganJ M Richman
Apr 2, 2016·Journal of Neurosurgery. Pediatrics·Alaina WhittonRene Olivares-Navarrete
Dec 21, 2007·Differentiation; Research in Biological Diversity·Anna K CoussensAngela van Daal
Mar 3, 2009·BMB Reports·Young-Mi ChaeJe-Yoel Cho
Jun 17, 2015·The Journal of Craniofacial Surgery·Deana S ShenaqRussell R Reid
Mar 1, 2012·Developmental Biology·Kellie Duench, Tamara A Franz-Odendaal
Mar 1, 2008·Expert Review of Endocrinology & Metabolism·Yan GuoHong-Wen Deng

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