PMID: 9437244Oct 1, 1994Paper

Bone-related matrix proteins expression in vitro and in vivo by marrow stromal cell line

Tissue & Cell
D BenayahuA Shamay

Abstract

MBA-1, a bone marrow stroma-derived cell line, was transplanted in an ectopic site and formed endochondral bone. The ossicle developed through stages of cell proliferation, differentiated into a zone of hypertrophy and formed a chondroid-like area which further developed to primary mineralized bone. We explored the expression of various matrix proteins by MBA-1 cells in vitro and in the ossicle formed in vivo. MBA-1 cells constitutively expressed mRNAs encoding for collagen I, non-collagenous proteins and alkaline phosphatase. RNA extracted from the ossicle formed by these cells was expressed in a different pattern. The in vivo maturation of MBA-1 cells was accompanied by low expression of mRNA for procollagen alpha 2(I) and a marked increase in osteonectin and osteopontin mRNA levels. Thus, the ability to follow expression of these genes through bone formation in vivo has been demonstrated.

References

Jul 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K IbarakiM F Young
Sep 1, 1991·Calcified Tissue International·D BenayahuS Wientroub
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·P BiancoP G Robey
Jan 1, 1990·Connective Tissue Research·M F YoungJ D Termine
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M J WeissH Harris

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Citations

Dec 18, 2001·Journal of Cellular Biochemistry·D BenayahuJ Issakov
Sep 8, 2004·Journal of Cellular Biochemistry·Irena ShurDafna Benayahu
Feb 13, 1997·Biochemical and Biophysical Research Communications·D BenayahuS Wientroub
Mar 26, 1998·Journal of Cellular Biochemistry·R Massas, D Benayahu
Oct 1, 1995·Journal of Cellular Biochemistry·D BenayahuS Wientroub

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