Bony engineering using time-release porous scaffolds to provide sustained growth factor delivery

The Journal of Craniofacial Surgery
Caroline SzpalskiStephen M Warren

Abstract

Microporous scaffolds designed to improve bony repair have had limited success; therefore, we sought to evaluate whether time-released porous scaffolds with or without recombinant bone morphogenetic protein 2 (rhBMP-2) could enhance stem cell osteoinduction. Custom-made 15/85 hydroxyapatite/β-tricalcium phosphate scaffolds were left empty (E) or filled with rhBMP-2 (E+), calcium sulfate (CS), or CS and rhBMP-2 (CS+). All scaffolds were placed in media and weighed daily. Conditioned supernatant was analyzed for rhBMP-2 and then used to feed human adipose-derived mesenchymal stem cells (ASCs). Adipose-derived mesenchymal stem cell ALP activity, OSTERIX expression, and bone nodule formation were determined. E scaffolds retained 97% (SD, 2%) of the initial weight, whereas CS scaffolds had a near-linear 30% (SD, 3%) decrease over 60 days. E+ scaffolds released 155 (SD, 5) ng of rhBMP-2 (77%) by day 2. In contrast, CS+ scaffolds released only 30 (SD, 2) ng (10%) by day 2, and the remaining rhBMP-2 was released over 20 days. Conditioned media from E+ scaffolds stimulated the highest ALP activity and OSTERIX expression in ACSs on day 2. However, after day 6, media from CS+ scaffolds stimulated the highest ALP activity and OSTERIX expre...Continue Reading

References

Dec 1, 1982·Journal of Dental Research·J F Piecuch
Feb 1, 1983·The Journal of Prosthetic Dentistry·H W DenissenB V Rejda
Nov 1, 1995·The Journal of Bone and Joint Surgery. American Volume·W W Tomford
Sep 3, 1999·The Orthopedic Clinics of North America·T BoyceN Scarborough
Mar 1, 1959·American Journal of Surgery·L F PELTIER
Sep 1, 2007·The Journal of the American Academy of Orthopaedic Surgeons·David J Hak
Mar 6, 2010·Recent Patents on Biotechnology·Nina ZippelEdda Tobiasch

❮ Previous
Next ❯

Citations

Apr 19, 2013·Gene Therapy·J LaylievS M Warren
Jun 6, 2012·Comparative and Functional Genomics·Laurence Wurth
Nov 19, 2013·International Journal of Oral and Maxillofacial Surgery·H KokemüllerN-C Gellrich
Sep 22, 2012·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Andrea S ZanettiDaniel J Hayes
Jul 26, 2018·Journal of Tissue Engineering and Regenerative Medicine·Nick TovarPaulo G Coelho
Jul 25, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lukasz WitekPaulo G Coelho
Nov 3, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Aránzazu MedieroBruce N Cronstein
Apr 30, 2019·Plastic and Reconstructive Surgery·Christopher D LopezPaulo G Coelho
Feb 13, 2018·The Journal of Surgical Research·Christopher D LopezPaulo G Coelho

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.