Abstract
Currently, there is no treatment strategy which can reverse the process of neuro-degeneration in progression of Alzheimer's disease (AD). Practically, it is desired to achieve and maintain high therapeutic doses in the brain, but it is hard due to selective permeability of the blood-brain barrier (BBB). In the present study, lactoferrin (Lf) was conjugated to polyamidoamine generation 3.0 (PAMAM G3.0) dendrimers for the effective delivery of rivastigmine (RIV) to the brain. Conjugation of PAMAM G3.0 with lactoferrin was confirmed by FT-IR, 1H NMR, and 2D-NMR spectroscopy as well as AFM techniques. Further, RIV was loaded into PAMAM G3.0 and PAMAM-Lf conjugates. RP-HPLC was used to quantify the drug loading and release as well. Spectroscopic analysis confirmed PAMAM-Lf conjugation, the size of the conjugate was 100.04 ± 3.1 nm, and after RIV loading, the size was increased up to 216.13 ± 2.3 nm. Atomic force microscopic results revealed that the root-mean-square roughness ( Rq) and surface roughness ( Ra) were 6.31 and 5.27 nm, respectively, along with other parameters, Skewness and Kurtosis, which were 0.522 and 2.50, respectively. In vitro drug release from the PAMAM-Lf-RIV conjugate was sustained up to more than 100 h, and th...Continue Reading
References
Nov 8, 1984·Nature·W A JefferiesD Y Mason
May 12, 1998·Acta Neurologica Scandinavica·N R CutlerP Zolnouni
Jul 31, 2001·CNS Drugs·B P Imbimbo
Apr 23, 2004·Biomedical Chromatography : BMC·Albert EnzAndre Dattler
Sep 17, 2004·Biomaterials·Eun Kyoung ParkYoung Moo Lee
Apr 27, 2007·Biomaterials·Payal AgrawalN K Jain
Oct 16, 2007·Biomaterials·Rongqin HuangYuanying Pei
Feb 23, 2008·Brain Research·Barnabas WilsonBhojraj Suresh
Oct 28, 2008·Bioconjugate Chemistry·Prateek SinghNarendra K Jain
Apr 28, 2009·International Journal of Clinical Practice·A KurzG Lefèvre
Dec 1, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Swati KoppuChristine Dufès
Dec 15, 2010·Trends in Cognitive Sciences·Masud Husain, Mitul A Mehta
Jun 10, 2011·International Journal of Pharmaceutics·Kaili HuXinguo Jiang
Dec 14, 2011·Cognitive Processing·M Antunes, G Biala
May 8, 2013·Therapeutic Delivery·Christine DufèsSukrut Somani
Jul 1, 2011·International Journal of Applied & Basic Medical Research·Uma A BhosaleRahul S Somani
Oct 24, 2014·Annual Review of Pharmacology and Toxicology·Jason M Lajoie, Eric V Shusta
May 21, 2015·Nanoscale Research Letters·Nikolaos Spyropoulos-AntonakakisAlkiviadis-Constantinos Cefalas
Sep 13, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Sukrut SomaniChristine Dufès
Mar 25, 2017·International Journal of Molecular Sciences·Bhairavi SrinageshwarJulien Rossignol
Dec 2, 2017·Molecular Pharmaceutics·Avinash GothwalUmesh Gupta
Citations
May 20, 2020·International Journal of Nanomedicine·Liang KongLan Cheng
Jul 20, 2020·Neurotoxicity Research·Sarathlal K CRajeev Taliyan
Nov 20, 2020·International Journal of Biological Macromolecules·Mona M Agwa, Sally Sabra
May 13, 2021·Journal of Dietary Supplements·Sreus A G NaiduA Satyanarayan Naidu
Sep 26, 2019·Bioconjugate Chemistry·Avinash GothwalUmesh Gupta
Jul 2, 2019·ACS Chemical Neuroscience·Avinash GothwalUmesh Gupta
Aug 17, 2019·ACS Chemical Neuroscience·Kowthavarapu Venkata KrishnaSunil Kumar Dubey