Boosting of enzymatic softwood saccharification by fungal GH5 and GH26 endomannanases

Biotechnology for Biofuels
Pernille von FreieslebenAnne S Meyer

Abstract

Softwood is a promising feedstock for lignocellulosic biorefineries, but as it contains galactoglucomannan efficient mannan-degrading enzymes are required to unlock its full potential. Boosting of the saccharification of pretreated softwood (Canadian lodgepole pine) was investigated for 10 fungal endo-β(1→4)-mannanases (endomannanases) from GH5 and GH26, including 6 novel GH26 enzymes. The endomannanases from Trichoderma reesei (TresMan5A) and Podospora anserina (PansMan26) were investigated with and without their carbohydrate-binding module (CBM). The pH optimum and initial rates of enzyme catalysed hydrolysis were determined on pure β-mannans, including acetylated and deacetylated spruce galactoglucomannan. Melting temperature (Tm) and stability of the endomannanases during prolonged incubations were also assessed. The highest initial rates on the pure mannans were attained by GH26 endomannanases. Acetylation tended to decrease the enzymatic rates to different extents depending on the enzyme. Despite exhibiting low rates on the pure mannan substrates, TresMan5A with CBM1 catalysed highest release among the endomannanases of both mannose and glucose during softwood saccharification. The presence of the CBM1 as well as the cata...Continue Reading

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Citations

Aug 26, 2020·Journal of Industrial Microbiology & Biotechnology·Heidi ØstbyAnikó Várnai
Mar 7, 2020·The Journal of Biological Chemistry·Fernanda MandelliMário Tyago Murakami
Oct 10, 2020·Critical Reviews in Biotechnology·Gaurav Singh Kaira, Mukesh Kapoor
Feb 6, 2019·Biochemical and Biophysical Research Communications·Gaurav Singh Kaira, Mukesh Kapoor
Apr 11, 2021·The Journal of Biological Chemistry·Marie Sofie MøllerBirte Svensson
Apr 30, 2019·International Journal of Biological Macromolecules·Gaurav Singh KairaMukesh Kapoor

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Methods Mentioned

BETA
ion exchange chromatography
differential scanning calorimetry
chips

Software Mentioned

SASjmp

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