Borate-fructose complex: A novel mediator for laccase and its new function for fructose determination

FEBS Letters
Chih-Yu ChengShuen-Fuh Lin

Abstract

Laccase and borate-fructose complex were investigated by coincidence in a solid-state fermentation of Edenia sp. TS-76 under fructose oxidase screening. Laccase was purified to homogeneity with a 34-fold purification and 32% yield. Fructose had no significant effect on laccase activity, whereas borate reduced laccase activity by 60-90%; conversely, the borate-fructose complex increased laccase activity by nearly fourfold at pH 7.5. The complex caused a shift in the optimal pH for laccase from 5.0 to 7.5 and served as a highly efficient mediator. Borate complexed with fructose provides an alternative, time-saving, and specific method for serum fructose determination.

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S S Tate, A Meister
Feb 10, 1976·Biochemistry·K W Smith, S L Johnson
Apr 1, 1996·Enzyme and Microbial Technology·S F LinY C Tsai
May 20, 1999·Journal of Microbiological Methods·U BamingerD Haltrich
Mar 1, 2003·Archives of Microbiology·Harald Claus
Oct 24, 2003·Antonie van Leeuwenhoek·Brenda ValderramaRafael Vazquez-Duhalt
Mar 31, 2004·The American Journal of Clinical Nutrition·George A BrayBarry M Popkin
Oct 12, 2004·FEBS Letters·Laura-Leena KiiskinenKristiina Kruus
Apr 7, 2005·Applied and Environmental Microbiology·Susana CamareroAngel T Martínez
Feb 14, 2006·FEMS Microbiology Reviews·Petr Baldrian
Apr 2, 2008·Applied and Environmental Microbiology·Nam-Hee KimDeok-Kun Oh
Oct 22, 2009·Cellular and Molecular Life Sciences : CMLS·Paola GiardinaGiovanni Sannia
Mar 12, 2010·Insect Biochemistry and Molecular Biology·Neal T Dittmer, Michael R Kanost
Jul 14, 2010·Archives of Microbiology·Benjamin OttoWerner Reisser

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Glycosides

Cardiac glycosides are a diverse family of naturally derived compounds that bind to and inhibit na+/k+-atpase. Discover the latest research on cardiac glycosides heres.

Related Papers

Biochimica Et Biophysica Acta
G MALMSTROMR MOSBACH
Emergency Medicine Journal : EMJ
Hannah Melville, Jonathan Costello
© 2021 Meta ULC. All rights reserved