Borate promotes the formation of a complex between legume AGP-extensin and Rhamnogalacturonan II and enhances production of Rhizobium capsular polysaccharide during infection thread development in Pisum sativum symbiotic root nodules

Plant, Cell & Environment
María RegueraL. Bolanos

Abstract

The capacity to bind to biomolecules is considered to be the basis for any physiological role of boron (B). Legume arabinogalactan protein-extensin (AGPE), a major component of the infection thread matrix of legume nodules is a potential B-ligand. Therefore, its role in infection threads development was investigated in Pisum sativum grown under B deficiency. Using the AGPE-specific antibody MAC265, immunochemical analysis revealed that a 175 kDa MAC265 antigen was abundant in +B but much weaker in -B nodule extracts. A B-dependent complex involving AGPE and rhamnogalacturonan II (RGII) could be co-purified using anti-RGII antiserum. Following fractionation of -B nodules, MAC265 antigens were mostly associated with the bacterial pellet. Immunogold staining confirmed that AGPE was closely associated with the surface of rhizobia in the lumen of threads in -B nodules whereas in +B nodules, AGPE was separated from the bacterial surface by a sheath of capsular polysaccharide. Interestingly, colonies of rhizobia grown in free-living culture without B developed low capsule production. Therefore, we propose that B could be important for apical growth of infection threads by strengthening thread wall through a B-dependent AGPE-RGII inter...Continue Reading

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Citations

Jan 23, 2016·The New Phytologist·Aleksandr GavrinElena E Fedorova
Jun 21, 2011·Plant Science : an International Journal of Experimental Plant Biology·Juan J Camacho-CristóbalAgustín González-Fontes
Jun 27, 2018·Journal of Experimental Botany·Romain CastilleuxMaïté Vicré
Dec 11, 2020·BMC Plant Biology·Sara Granado-RodríguezMaria Reguera
Jun 4, 2021·Frontiers in Plant Science·Dagmar HromadováEdita Tylová

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