Border Cell Migration: A Model System for Live Imaging and Genetic Analysis of Collective Cell Movement

Methods in Molecular Biology
Mohit PrasadDenise Montell

Abstract

Border cell migration in the Drosophila ovary has emerged as a genetically tractable model for studying collective cell movement. Over many years border cell migration was exclusively studied in fixed samples due to the inability to culture stage 9 egg chambers in vitro. Although culturing late-stage egg chambers was long feasible, stage 9 egg chambers survived only briefly outside the female body. We identified culture conditions that support stage 9 egg chamber development and sustain complete migration of border cells ex vivo. This protocol enables one to compare the dynamics of egg chamber development in wild-type and mutant egg chambers using time-lapse microscopy and taking advantage of a multiposition microscope with a motorized imaging stage. In addition, this protocol has been successfully used in combination with fluorescence resonance energy transfer biosensors, photo-activatable proteins, and pharmacological agents and can be used with wide-field or confocal microscopes in either an upright or an inverted configuration.

Citations

Feb 23, 2019·Cell Death & Disease·Eun-Kyoung BreuerSaverio Gentile
Apr 5, 2019·Nature Communications·Fernanda BajancaEric Theveneau
Feb 28, 2020·Cell Death & Disease·Josephine VolovetzJustin D Lathia
Oct 30, 2020·Frontiers in Cell and Developmental Biology·Matthew P BostockVilaiwan M Fernandes
Nov 23, 2019·Current Opinion in Cell Biology·Lavinia CapuanaSandrine Etienne-Manneville
May 18, 2021·Cells & Development·Brenda Canales Coutiño, Roberto Mayor
Aug 31, 2021·Cells & Development·Brenda Canales Coutiño, Roberto Mayor

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