Bordetella holmesii Contamination of Platelet Concentrates: Revisiting the Definition of a Positive Culture

Journal of Clinical Microbiology
Marc CloutierLouis Thibault

Abstract

Bacterial contamination remains the most important infectious risk of platelet transfusion. After an initially positive result, a second test is performed on the blood products and the initial culture bottle to confirm the contamination. Based on the blood center's decision algorithm used, results can be either confirmed negative, positive, or indeterminate, or be unconfirmed or discordant. Here, we report the first cases of platelet concentrates contaminated with Bordetella holmesii The in vitro growth characteristics of this unusual contaminant in platelet concentrate were investigated. Two B. holmesii strains isolated from platelet concentrates, as well as a control strain (Serratia marcescens), were spiked into platelet concentrates (PCs) at 1 and 10 CFU/ml. PCs were stored at 20 to 24°C under agitation. Samples were collected on days 2, 3, 4, and 7 for colony count and for bacterial screening using the BacT/Alert 3D system. Two PCs were detected as being positive for B. holmesii However, recultures were negative. In vitro, B. holmesii did not grow but remained detectable in PCs. Its viability diminished rapidly in contact with human plasma. Upon screening using the BacT/Alert 3D system, the majority of products spiked with...Continue Reading

References

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Apr 12, 2014·The Lancet Infectious Diseases·Laure F PittetKlara M Posfay-Barbe
Feb 9, 2017·Frontiers in Microbiology·Illiassou Hamidou SoumanaEric T Harvill

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Citations

Sep 21, 2018·Journal of Clinical Microbiology·Eric A Gehrie

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