Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

International Journal of Molecular Sciences
Valerie BouchezM Caroff

Abstract

Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography-mass spectrometry (GC-MS), and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2' position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the is...Continue Reading

References

Aug 1, 1992·Scientific American·E T Rietschel, H Brade
Oct 1, 1970·Journal of General Microbiology·D W Stainer, M J Scholte
Jan 1, 1995·Journal of Clinical Microbiology·R S WeyantD J Brenner
Aug 1, 1994·Journal of Bacteriology·M CaroffD Karibian
Nov 5, 1999·Rapid Communications in Mass Spectrometry : RCM·D KaribianM Caroff
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E NjamkepoN Guiso
Feb 24, 2001·Cellular Microbiology·T A Flak, W E Goldman
Jun 3, 2005·Journal of Lipid Research·Asmaa El HamidiMartine Caroff
Nov 20, 2008·Journal of Lipid Research·Asmaa El HamidiMartine Caroff
Nov 6, 2010·The Journal of Infectious Diseases·Nico MarrRachel C Fernandez
Jun 24, 2011·Rapid Communications in Mass Spectrometry : RCM·Imane Chafchaouni-MoussaouiMartine Caroff
Jul 19, 2011·Journal of Bacteriology·I MacArthurA Preston
Nov 22, 2011·FEMS Immunology and Medical Microbiology·Frits R MooiAudrey J King
Mar 20, 2012·Journal of Clinical Microbiology·Carolina MirandaPatricia García
Jun 20, 2012·Emerging Infectious Diseases·Hajime KamiyaNobuhiko Okabe
Aug 10, 2012·Epidemiology and Infection·D BotteroD F Hozbor
Aug 14, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A TizolovaS Guillot
Oct 23, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Loren RodgersMary Diorio
Mar 22, 2013·Genome Announcements·Kathleen M TattiM Lucia Tondella
Apr 27, 2013·Pathogens and Disease·Paul J PlanetAdam J Ratner
Sep 21, 2013·Emerging Infectious Diseases·Liem Binh Luong NguyenNicole Guiso
Jul 19, 2014·Expert Review of Vaccines·Nicole Guiso, Nicolas Hegerle
Jun 13, 2015·Expert Review of Anti-infective Therapy·Laure F Pittet, Klara M Posfay-Barbe
May 27, 2016·Microbiology Spectrum·Laure F Pittet, Klara M Posfay-Barbe

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