Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction.

Infection and Immunity
Fabiana SpensieriC M Ausiello

Abstract

Bordetella pertussis, the causative agent of whooping cough, possesses an array of virulence factors, including adenylate cyclase toxin (ACT), relevant in the establishment of infection. Here we better define the impact of cyclic AMP (cAMP) intoxication due to the action of ACT on dendritic cell (DC)-driven immune response, by infecting monocyte-derived DC (MDDC) with an ACT-deficient B. pertussis mutant (ACT- 18HS19) or its parental strain (WT18323). Both strains induced MDDC maturation and antigen-presenting cell functions; however, only ACT- 18HS19 infected MDDC-induced production of interleukin-12 (IL-12) p70. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. Addition of the cAMP analogous D-butyril-cAMP (D-cAMP) abolished IL-12 p70 production and IL-12 p35 expression in ACT- 18HS19-infected MDDC. ACT- 18HS19 infection induced the expression of the transcription factors interferon regulatory factor 1 (IRF-1) and IRF-8 and of beta interferon, involved in IL-12 p35 regul...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·R M Steinman
Jul 11, 1998·The Journal of Biological Chemistry·M GrayE Hewlett
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G CarraG Trinchieri
Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·I M WangK Ozato
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M Delgado, D Ganea
Feb 15, 2001·Current Opinion in Microbiology·C LochtF Jacob-Dubuisson
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S CorintiG Girolomoni
Jul 11, 2001·Microbes and Infection·K H Mills
Sep 10, 2002·Trends in Microbiology·Maria Rescigno
Apr 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kyle I HappelJay K Kolls
Mar 25, 2005·The New England Journal of Medicine·Erik L Hewlett, Kathryn M Edwards
Jul 7, 2005·Nature Reviews. Immunology·Christopher A Hunter
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aoife P BoydKingston H G Mills
Nov 18, 2005·Trends in Immunology·Brent S McKenzieDaniel J Cua

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Citations

Aug 21, 2013·Archivum Immunologiae Et Therapiae Experimentalis·Giorgio FedeleClara Maria Ausiello
Jun 22, 2012·Mucosal Immunology·R HiggsK H G Mills
Oct 31, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patricia J Dubin, Jay K Kolls
Jun 12, 2010·Expert Opinion on Therapeutic Targets·Mario M D'EliosAmedeo Amedei
Aug 5, 2014·Expert Review of Vaccines·Peter SeboJiri Masin
Feb 13, 2016·Frontiers in Microbiology·Ana do ValeSandra Sousa
May 4, 2013·British Journal of Pharmacology·M GschwandtnerR Gutzmer
Sep 9, 2015·Pathogens and Disease·Jolanda BrummelmanKingston H G Mills
Aug 6, 2015·Pathogens and Disease·Giorgio FedeleClara Maria Ausiello
Apr 17, 2016·Current Opinion in Cell Biology·Jeffrey M Williams, Billy Tsai
Jan 6, 2011·FEMS Microbiology Reviews·Daan de GouwFrits R Mooi
Jan 10, 2013·Cytokine·Roelof A de PausEsther van de Vosse
Jan 8, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kirsten M HooperDoina Ganea
Mar 14, 2018·The British Journal of Dermatology·N LaceyF C Powell
Nov 12, 2014·Infection and Immunity·Katina SchinnerlingThomas Schneider
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xisheng WangBuka Samten
Jul 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maria NassoClara Maria Ausiello
Mar 25, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giorgio FedeleClara Maria Ausiello
Aug 21, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xisheng WangBuka Samten
May 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Edith JasnyRalf Ignatius
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tanzilya KhayrullinaDoina Ganea
Jun 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Giorgio FedeleClara M Ausiello
Jun 13, 2021·Infectious Diseases and Therapy·Denis Macina, Keith E Evans

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