Boric acid increases the expression levels of human anion exchanger genes SLC4A2 and SLC4A3

Genetics and Molecular Research : GMR
F Akbas, Z Aydin

Abstract

Boron is an important micronutrient in plants and animals. The role of boron in living systems includes coordinated regulation of gene expression, growth and proliferation of higher plants and animals. There are several well-defined genes associated with boron transportation and tolerance in plants and these genes show close homology with human anion exchanger genes. Mutation of these genes also characterizes some genetic disorders. We investigated the toxic effects of boric acid on HEK293 cells and mRNA expression of anion exchanger (SLC4A1, SLC4A2 and SLC4A3) genes. Cytotoxicity of boric acid at different concentrations was tested by using the methylthiazolyldiphenyl-tetrazolium bromide assay. Gene expression profiles were examined using quantitative real-time PCR. In the HEK293 cells, the nontoxic upper concentration of boric acid was 250 μM; more than 500 μM caused cytotoxicity. The 250 μM boric acid concentration increased gene expression level of SLC4A2 up to 8.6-fold and SLC4A3 up to 2.6-fold, after 36-h incubation. There was no significant effect of boric acid on SLC4A1 mRNA expression levels.

References

Jan 1, 1985·American Journal of Industrial Medicine·J R Landolph
Nov 1, 1994·Environmental Health Perspectives·F H Nielsen
Jan 1, 1996·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·N TillyB Glimelius
Jan 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J F MedinaJ Prieto
Jan 7, 2000·Biochemical and Biophysical Research Communications·J F MedinaJ Prieto
May 29, 2000·The Journal of Biological Chemistry·M F RomeroC M Sciortino
Sep 8, 2001·American Journal of Physiology. Cell Physiology·A K StewartS L Alper
Feb 5, 2002·Annual Review of Physiology·Seth L Alper
Nov 26, 2002·Nature·Junpei TakanoToru Fujiwara
Feb 13, 2003·The Biochemical Journal·Jocelyne FujinagaJoseph R Casey
Apr 23, 2003·Critical Reviews in Food Science and Nutrition·Tara A Devirian, Stella L Volpe
Jan 15, 2004·Pflügers Archiv : European journal of physiology·Michael F RomeroWalter F Boron
Apr 9, 2005·American Journal of Physiology. Cell Physiology·Teresa ShermanKeith Nehrke
Oct 22, 2005·Experimental Physiology·Seth L Alper
Dec 1, 2007·Science·Kyoko MiwaToru Fujiwara
Jan 23, 2008·Ophthalmic Plastic and Reconstructive Surgery·Juan A Aviñó-MartínezMarino Blanes
Dec 9, 2008·The Journal of Antimicrobial Chemotherapy·Francesco De SetaBryan Larsen
May 19, 2009·The Journal of Experimental Biology·Seth L Alper
Jul 10, 2009·The Journal of Biological Chemistry·Ivan A LopezIra Kurtz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.