Borna disease virus possesses an NF-ĸB inhibitory sequence in the nucleoprotein gene

Scientific Reports
Akiko MakinoKeizo Tomonaga

Abstract

Borna disease virus (BDV) has a non-segmented, negative-stranded RNA genome and causes persistent infection in many animal species. Previous study has shown that the activation of the IκB kinase (IKK)/NF-κB pathway is reduced by BDV infection even in cells expressing constitutively active mutant IKK. This result suggests that BDV directly interferes with the IKK/NF-κB pathway. To elucidate the mechanism for the inhibition of NF-κB activation by BDV infection, we evaluated the cross-talk between BDV infection and the NF-κB pathway. Using Multiple EM for Motif Elicitation analysis, we found that the nucleoproteins of BDV (BDV-N) and NF-κB1 share a common ankyrin-like motif. When THP1-CD14 cells were pre-treated with the identified peptide, NF-κB activation by Toll-like receptor ligands was suppressed. The 20S proteasome assay showed that BDV-N and BDV-N-derived peptide inhibited the processing of NF-κB1 p105 into p50. Furthermore, immunoprecipitation assays showed that BDV-N interacted with NF-κB1 but not with NF-κB2, which shares no common motif with BDV-N. These results suggest BDV-N inhibits NF-κB1 processing by the 20S proteasome through its ankyrin-like peptide sequence, resulting in the suppression of IKK/NF-κB pathway acti...Continue Reading

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Citations

May 24, 2016·Animal Health Research Reviews·Ian TizardSusan Payne
Dec 14, 2018·Journal of Virology·Shohei KojimaKeizo Tomonaga
Jan 11, 2016·Wiley Interdisciplinary Reviews. RNA·Binbin DingMingzhou Chen
Feb 6, 2017·Animal Health Research Reviews·Ian TizardSusan Payne
Sep 11, 2020·Scandinavian Journal of Immunology·Xuejiao LiFengmin Zhang

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Methods Mentioned

BETA
immunoprecipitation assay
co-immunoprecipitation
FCS

Software Mentioned

MEME
MEME analysis

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