Bornyl caffeate induces apoptosis in human breast cancer MCF-7 cells via the ROS- and JNK-mediated pathways

Acta Pharmacologica Sinica
Chuan-bin YangJian-hui Rong

Abstract

The purpose of the present study was to investigate the anticancer activity of bornyl caffeate in the human breast cancer cell line MCF-7. The cell viability was determined using the MTT assay, and apoptosis was initially defined by monitoring the morphology of the cell nuclei and staining an early apoptotic biomarker with Annexin V-FITC. The mitochondrial membrane potential was visualized by JC-1 under fluorescence microscopy, whereas intracellular reactive oxygen species (ROS) were assessed by flow cytometry. The expression of apoptosis-associated proteins was determined by Western blotting analysis. Bornyl caffeate induced apoptosis in MCF-7 cells in a dose- and time-dependent manner. Consistently, bornyl caffeate increased Bax and decreased Bcl-xl, resulting in the disruption of MMP and subsequent activation of caspase-3. Moreover, bornyl caffeate triggered the formation of ROS and the activation of the mitogen-activated protein (MAP) kinases p38 and c-Jun N-terminal kinase (JNK). Antioxidants attenuated the activation of MAP kinase p38 but barely affected the activation of JNK. Importantly, the cytotoxicity of bornyl caffeate was partially attenuated by scavenging ROS and inhibited by MAP kinases and caspases. The present ...Continue Reading

References

Feb 5, 1998·JAMA : the Journal of the American Medical Association·S W Hetts
Apr 30, 1998·Advances in Cancer Research·T S LewisN G Ahn
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Jun 25, 1999·Cell Death and Differentiation·C Borner, L Monney
Nov 2, 1999·Biochemical and Biophysical Research Communications·Y Ishikawa, M Kitamura
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·T MinaminoN Terada
Dec 28, 1999·Annual Review of Cell and Developmental Biology·I BudihardjoX Wang
Mar 31, 2000·Experimental Cell Research·M BamfordR Brown
Mar 31, 2000·Experimental Cell Research·S H Kaufmann, W C Earnshaw
May 12, 2000·Critical Care Medicine·T ObataM B Yaffe
Nov 4, 2000·Science·L ZhangB Vogelstein
Feb 24, 2001·Bioorganic & Medicinal Chemistry·B EtzenhouserC D Selassie
Mar 10, 2001·Nature·L Chang, M Karin
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·M Leist, M Jäättelä
May 7, 2002·EMBO Reports·Moran BenharAlexander Levitzki
May 15, 2003·Journal of Agricultural and Food Chemistry·George HsiaoJoen-Rong Sheu
Apr 13, 2004·Oncogene·Teiji Wada, Josef M Penninger
Dec 29, 2004·Free Radical Research·Fernanda M da CunhaJoão B Calixto
Mar 9, 2005·Cancer Research·Hyung Woo YimYung-Jue Bang
Dec 6, 2005·Biological & Pharmaceutical Bulletin·Mayuko UjibeMasaaki Ishikawa
Mar 17, 2006·Journal of Medicinal Chemistry·Thomas SteinbrecherAndreas Labahn
Jan 27, 2007·Planta medica·E MaldonadoA L Pérez-Castorena
Feb 10, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·John P Fruehauf, Frank L Meyskens
Jun 26, 2007·Phytochemistry·Yu-Chang ChenIh-Sheng Chen
Aug 29, 2007·Acta Pharmacologica Sinica·Ki Han KwonAh-Ng Tony Kong
Oct 28, 2008·Bioorganic & Medicinal Chemistry Letters·Chun-nian XiaWei-xiao Hu

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Citations

May 29, 2015·Arquivos De Gastroenterologia·Keli Cristina Simões da SilveiraMarilene Porawski
Dec 29, 2018·Molecular Medicine Reports·Lei WuYouhong Jiang
Dec 10, 2019·International Journal of Medicinal Chemistry·Merly de Armas-RicardOney Ramírez-Rodríguez
Mar 13, 2021·Pharmacological Reports : PR·Kamal S AbdelrahmanMohamed Abdel-Aziz
May 1, 2021·International Journal of Molecular Sciences·Stephanie Cristine Hepp RehfeldtMárcia Inês Goettert

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