Boronate affinity nanoparticles for RNA isolation

Materials Science & Engineering. C, Materials for Biological Applications
Aykut ToprakSinan Akgöl

Abstract

In this presented paper, boronic acid incorporated poly(HEMA) based nanoparticles were synthesized for RNA adsorption. For this purpose, poly(HEMA) nanoparticles were synthesized by using the surfactant free emulsion polymerization technique. Then, nanoparticles were modified with 3-(2-imidazoline-1-yl)propyl(triethoxysilane) (IMEO) and functionalized with phenylboronic acid (PBA). Prepared nanoparticles were characterized with SEM, FTIR and zeta-size. Optimum RNA adsorption conditions were investigated with different pHs, temperatures and initial RNA concentrations in order to determine the maximum RNA adsorption onto poly(HEMA)-IMEO-PBA nanoparticles. It was also studied that, synthesized nanoparticles could be used for 5 successive reuses and adsorption capacity of the nanoparticles decreased only about 5% at the end of the 5 cycles.

References

Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Apr 26, 2005·Journal of Colloid and Interface Science·Madhuri MandalTarasankar Pal
Jun 6, 2007·RNA·Robert T Batey, Jeffrey S Kieft
Sep 13, 2008·Colloids and Surfaces. B, Biointerfaces·Nevra OztürkAdil Denizli
Nov 1, 2007·ACS Nano·Jie BaoYadong Li
Feb 26, 2009·Cell·Phillip A Sharp
Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Ceren TürkcanAdil Denizli

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Citations

Mar 24, 2016·Journal of Separation Science·Kazım Köse, Lokman Uzun
May 16, 2017·Journal of Biomaterials Science. Polymer Edition·Ceren Türkcan KayhanAdil Denizli
May 1, 2019·Journal of Biomaterials Science. Polymer Edition·Ceren TürkcanSinan Akgöl
Jul 7, 2018·Beilstein Journal of Nanotechnology·Tatiana Yu SergeevaAlexander I Konovalov
May 30, 2021·Biotechnology and Bioengineering·Sinan AkgölKevser Kuşat

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