Borrelia burgdorferi enzyme-linked immunosorbent assay for discrimination of OspA vaccination from spirochete infection.

Journal of Clinical Microbiology
Y Q ZhangD H Persing

Abstract

Recombinant Lyme disease vaccines based on purified preparations of outer surface protein A (OspA) have been shown to be effective in preventing transmission of Borrelia burgdorferi in experimental animal models and are now being tested in humans. Since the most widely used screening tests for Lyme disease are based on a whole-cell sonicate of B. burgdorferi, serologic false positivity in vaccinated persons could result from reactivity to OspA within the antigen preparation. In order to avoid serologic false positivity in vaccinated subjects, we developed an immunoassay based on a low-passage-number, naturally occurring variant of B. burgdorferi which lacks the plasmid encoding OspA and OspB. The use of an antigen preparation derived from this organism provided sensitive and specific detection of B. burgdorferi seropositivity in experimental animals and in human Lyme disease cases. The OspA-B-negative enzyme-linked immunosorbent assay (ELISA) also appeared to be capable of discriminating the vaccinated state from vaccine failure and natural infection in experimental animals. Sera from human subjects participating in a vaccine trial gave false-positive results with an ELISA based on an OspA-containing strain, but no such reactiv...Continue Reading

References

Mar 1, 1992·Infection and Immunity·E FikrigR A Flavell
Jun 1, 1992·The Journal of Infectious Diseases·E FikrigR A Flavell
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E FikrigR A Flavell
Jun 1, 1985·Mayo Clinic Proceedings·L E MertzJ A Katzmann
Mar 31, 1983·The New England Journal of Medicine·A C SteereS E Malawista
Mar 31, 1983·The New England Journal of Medicine·J L BenachR A Kaslow
Jun 18, 1982·Science·W BurgdorferJ P Davis
May 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J S Dumler, J S Bakken
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·T G SchwanP A Rosa
Feb 1, 1995·Journal of Clinical Microbiology·S M EngstromR C Johnson
Apr 24, 1995·The American Journal of Medicine·L A Magnarelli
Jun 1, 1995·Infection and Immunity·S W BartholdD H Persing
Feb 2, 1995·The New England Journal of Medicine·D H PersingP A Conrad
Mar 1, 1995·The Journal of Infectious Diseases·M A GerberS J Padula
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·A C Steere
Feb 1, 1993·The Journal of Infectious Diseases·F DresslerA C Steere
Apr 6, 1996·Lancet·G P WormserM Aguero-Rosenfeld

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Citations

Mar 15, 2002·Current Allergy and Asthma Reports·A R Marques
Jul 29, 2000·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P J MolloyL H Sigal
Oct 19, 1999·Journal of Clinical Microbiology·M E Aguero-RosenfeldG P Wormser
Nov 24, 1999·Journal of Clinical Microbiology·M B LawrenzS J Norris
May 1, 1997·Journal of Clinical Microbiology·C P KolbertD H Persing
May 2, 2002·The Medical Clinics of North America·Mary Elizabeth Wilson
Jul 11, 2001·Mayo Clinic Proceedings·G A Poland

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