Abstract
Canine synovial membrane explants were exposed to high- or low-passage Borrelia burgdorferi for 3, 6, 12, and 24 h. Spirochetes received no treatment, were UV light irradiated for 16 h, or were sonicated prior to addition to synovial explant cultures. In explant tissues, mRNA levels for the proinflammatory cytokines tumor necrosis factor alpha (TNF-alpha), interleukin-1alpha (IL-1alpha), IL-1beta, and IL-8 were surveyed semiquantitatively by reverse transcription-PCR. Culture supernatants were examined for numbers of total and motile (i.e., viable) spirochetes, TNF-like and IL-1-like activities, polymorphonuclear neutrophil (PMN) chemotaxis-inducing activities, and IL-8. During exposure to synovial explant tissues, the total number of spirochetes in the supernatants decreased gradually by approximately 30%, and the viability also declined. mRNAs for TNF-alpha, IL-1alpha, IL-1beta, and IL-8 were up-regulated in synovial explant tissues within 3 h after infection with untreated or UV light-irradiated B. burgdorferi, and mRNA levels corresponded to the results obtained with bioassays. During 24 h of coincubation, cultures challenged with untreated or UV light-irradiated spirochetes produced similar levels of TNF-like and IL-1-like...Continue Reading
References
Sep 1, 1992·Infection and Immunity·K B KenefickC J Czuprynski
Sep 16, 1992·Biochemical and Biophysical Research Communications·T TanakaH Hirai
Apr 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R PoratC A Dinarello
Mar 1, 1992·Infection and Immunity·D L Defosse, R C Johnson
Jun 1, 1991·Arthritis and Rheumatism·K GeorgilisM S Klempner
Dec 1, 1990·Veterinary Immunology and Immunopathology·M K Thomsen, H K Thomsen
Nov 1, 1990·Infection·V Preac MursicP Mehraein
Jan 1, 1990·International Immunology·H YsselG Peltz
Jan 1, 1990·Agents and Actions·M K Thomsen
Mar 1, 1989·Journal of Biochemistry·T NishidaY Hirai
Aug 31, 1989·The New England Journal of Medicine·A C Steere
Jan 1, 1989·Journal of Basic Microbiology·D HulínskáJ Herzogová
Oct 1, 1988·Clinical Microbiology Reviews·A G Barbour
Apr 1, 1988·Arthritis and Rheumatism·A C SteereE C Butcher
Sep 26, 1988·Nucleic Acids Research·S R LeongP W Gray
Feb 27, 1987·Biochemical and Biophysical Research Communications·T NishidaY Hirai
Dec 1, 1995·Infection and Immunity·L C LimR F Schell
Apr 1, 1995·Infection and Immunity·L C LimR F Schell
Oct 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A P van DamJ Dankert
Sep 15, 1993·Gene·J IshikawaK Suzuki
Jul 1, 1994·Infection and Immunity·L C LimR F Schell
Jan 16, 1993·Lancet·L C MillerA C Steere
Feb 1, 1994·The Journal of Veterinary Medical Science·K YamashitaT Kotani
Feb 1, 1994·Infection and Immunity·K F TaiJ J Weis
Sep 1, 1993·Infection and Immunity·Y Ma, J J Weis
Mar 1, 1993·Journal of Leukocyte Biology·K Mulder, I G Colditz
Nov 1, 1993·Infection and Immunity·L EckmannJ Fierer
Jan 4, 1993·Journal of Immunological Methods·M G Stevens, S C Olsen
Mar 1, 1993·The Journal of Infectious Diseases·M J AppelB A Summers
May 1, 1993·The Journal of Infectious Diseases·K B KenefickR F Schell
Oct 1, 1995·Journal of Clinical Microbiology·R K StraubingerM J Appel
Sep 1, 1996·Infection and Immunity·M V NorgardJ D Radolf
May 1, 1996·Medical Microbiology and Immunology·J SchulzeV Brade
Oct 1, 1996·European Journal of Immunology·H KniggeR Wallich
Apr 1, 1997·Infection and Immunity·M J BurnsM B Furie
Apr 1, 1997·Infection and Immunity·R K StraubingerM J Appel
Jan 1, 1993·Mediators of Inflammation·E MayatepekM Maiwald
Citations
Oct 3, 2001·Cellular Microbiology·L NeffD Wachsmann
Mar 5, 2008·Antimicrobial Agents and Chemotherapy·Emir HodzicStephen W Barthold
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guiqing WangIra Schwartz
Dec 5, 2008·Clinical and Vaccine Immunology : CVI·Rhonda L LaFleurRonald F Schell
Mar 24, 2007·FEMS Immunology and Medical Microbiology·Jens KnauerReinhard K Straubinger
Apr 9, 2001·Infection and Immunity·V ThomasE Fikrig
Apr 22, 2020·Parasites & Vectors·Liucun LiangReinhard K Straubinger
Mar 20, 2010·Clinical and Vaccine Immunology : CVI·Rhonda L LaFleurRonald F Schell
Mar 7, 2013·Journal of Veterinary Emergency and Critical Care·Meryl P Littman
Jul 17, 2019·Infection and Immunity·Emir HodzicEdlin Escobar
Mar 20, 2016·Microbes and Infection·Tricia A Van LaarJ Seshu
Feb 24, 2001·Arthritis and Rheumatism·J K FranzA Krause
Aug 24, 1999·Gene·A F StraubingerR K Straubinger