PMID: 9423865Jan 10, 1998Paper

Borrelia burgdorferi induces the production and release of proinflammatory cytokines in canine synovial explant cultures

Infection and Immunity
R K StraubingerM J Appel

Abstract

Canine synovial membrane explants were exposed to high- or low-passage Borrelia burgdorferi for 3, 6, 12, and 24 h. Spirochetes received no treatment, were UV light irradiated for 16 h, or were sonicated prior to addition to synovial explant cultures. In explant tissues, mRNA levels for the proinflammatory cytokines tumor necrosis factor alpha (TNF-alpha), interleukin-1alpha (IL-1alpha), IL-1beta, and IL-8 were surveyed semiquantitatively by reverse transcription-PCR. Culture supernatants were examined for numbers of total and motile (i.e., viable) spirochetes, TNF-like and IL-1-like activities, polymorphonuclear neutrophil (PMN) chemotaxis-inducing activities, and IL-8. During exposure to synovial explant tissues, the total number of spirochetes in the supernatants decreased gradually by approximately 30%, and the viability also declined. mRNAs for TNF-alpha, IL-1alpha, IL-1beta, and IL-8 were up-regulated in synovial explant tissues within 3 h after infection with untreated or UV light-irradiated B. burgdorferi, and mRNA levels corresponded to the results obtained with bioassays. During 24 h of coincubation, cultures challenged with untreated or UV light-irradiated spirochetes produced similar levels of TNF-like and IL-1-like...Continue Reading

References

Sep 16, 1992·Biochemical and Biophysical Research Communications·T TanakaH Hirai
Apr 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R PoratC A Dinarello
Mar 1, 1992·Infection and Immunity·D L Defosse, R C Johnson
Jun 1, 1991·Arthritis and Rheumatism·K GeorgilisM S Klempner
Dec 1, 1990·Veterinary Immunology and Immunopathology·M K Thomsen, H K Thomsen
Mar 1, 1989·Journal of Biochemistry·T NishidaY Hirai
Aug 31, 1989·The New England Journal of Medicine·A C Steere
Jan 1, 1989·Journal of Basic Microbiology·D HulínskáJ Herzogová
Oct 1, 1988·Clinical Microbiology Reviews·A G Barbour
Sep 26, 1988·Nucleic Acids Research·S R LeongP W Gray
Feb 27, 1987·Biochemical and Biophysical Research Communications·T NishidaY Hirai
Oct 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A P van DamJ Dankert
Feb 1, 1994·The Journal of Veterinary Medical Science·K YamashitaT Kotani
Mar 1, 1993·Journal of Leukocyte Biology·K Mulder, I G Colditz
Mar 1, 1993·The Journal of Infectious Diseases·M J AppelB A Summers
May 1, 1993·The Journal of Infectious Diseases·K B KenefickR F Schell
Jan 1, 1993·Mediators of Inflammation·E MayatepekM Maiwald

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Citations

Mar 5, 2008·Antimicrobial Agents and Chemotherapy·Emir HodzicStephen W Barthold
Jun 5, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guiqing WangIra Schwartz
Apr 22, 2020·Parasites & Vectors·Liucun LiangReinhard K Straubinger
Mar 20, 2010·Clinical and Vaccine Immunology : CVI·Rhonda L LaFleurRonald F Schell
Mar 7, 2013·Journal of Veterinary Emergency and Critical Care·Meryl P Littman

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