Borrelia burgdorferi lipoprotein BmpA activates pro-inflammatory responses in human synovial cells through a protein moiety.

Microbes and Infection
Xiuli YangUtpal Pal

Abstract

Borrelia burgdorferi invasion of mammalian joints results in genesis of Lyme arthritis. Other than spirochete lipids, existence of protein antigens, which are abundant in joints and participate in B. burgdorferi-induced host inflammatory response, is unknown. Here, we report that major products of the B. burgdorferi basic membrane protein (bmp) A/B operon that are induced in murine and human joints, possess inflammatory properties. Compared to the wild type B. burgdorferi, an isogenic bmpA/B mutant induced significantly lower levels of pro-inflammatory cytokines TNF-alpha and IL-1beta in cultured human synovial cells, which could be restored using bmpA/B-complemented mutants, and more directly, upon addition of recombinant BmpA, but not BmpB or control spirochete proteins. Non-lipidated and lipidated versions of BmpA induced similar levels of cytokines, and remained unaffected by treatment with lipopolysaccharide inhibitor, polymyxin B. The bmpA/B mutant was also impaired in the induction of NF-kappaB and p38 MAP kinase signaling pathways in synovial cells, which were activated by non-lipidated BmpA. These results show that a protein moiety of BmpA can induce cytokine responses in synovial cells via activation of the NF-kappaB ...Continue Reading

References

Aug 23, 2000·The Journal of Clinical Investigation·U PalE Fikrig
Mar 26, 2004·Nature Reviews. Immunology·Allen C Steere, Lisa Glickstein
Aug 10, 2004·The Journal of Biological Chemistry·Endre Kiss-TothSteven K Dower
Jan 2, 2008·The Journal of Experimental Medicine·Utpal PalErol Fikrig
Jan 4, 2008·The Journal of Infectious Diseases·Utpal PalErol Fikrig

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Citations

Apr 9, 2010·Current Opinion in Rheumatology·Bettina Panagiota Iliopoulou, Brigitte T Huber
Aug 26, 2009·Infection and Immunity·Ashutosh VermaBrian Stevenson
Oct 1, 2015·Expert Review of Vaccines·Chih-Hsiang LengPele Chong
Jun 16, 2010·FEMS Microbiology Letters·Anton V BryksinFelipe C Cabello
Mar 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chris M OlsonJuan Anguita
Nov 11, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Quentin Bernard, Linden T Hu

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