Both decrease in ACL1 gene expression and increase in ICL1 gene expression in marine-derived yeast Yarrowia lipolytica expressing INU1 gene enhance citric acid production from inulin.

Marine Biotechnology
Xiao-Yan LiuZhen-Ming Chi

Abstract

In this study, some of the ATP-citrate lyase genes (ACL1) were deleted and the copy number of the iso-citrate lyase gene (ICL1) was increased in the marine-derived yeast Yarrowia lipolytica SWJ-1b displaying the recombinant inulinase. It was found that lipid content and iso-citric acid in the transformant 30 obtained were greatly reduced and citric acid production was greatly enhanced. It was also found that the ACL1 gene expression and ATP-citrate lyase activity in the transformant 30 were declined and the ICL1 gene expression and iso-citrate lyase activity were promoted. During the 2-l fermentation, 84.0 g/l of citric acid and 1.8 g/l of iso-citric acid in the fermented medium were attained from 10.0 % of inulin by the transformant 30 within 214 h. The results showed that only 0.36 % of the residual reducing sugar and 1.0 % of the residual total sugar were left in the fermented medium, suggesting that 89.6 % of the total sugar was used for citric acid production and cell growth by the transformant 30.

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Citations

Aug 8, 2015·Biotechnology Advances·Hu-Hu LiuHe Huang
Jan 10, 2016·Current Opinion in Biotechnology·Yun Chen, Jens Nielsen
Mar 16, 2013·Critical Reviews in Microbiology·Marizeth GroenewaldMarkus Wyss
May 8, 2015·Applied Microbiology and Biotechnology·Catherine Madzak
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Nov 14, 2018·Applied Microbiology and Biotechnology·Wei HuJi-Hong Chen
Jan 8, 2021·Natural Product Reports·Yang LiXiulai Chen

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