Both forward and reverse TCA cycles operate in green sulfur bacteria.

The Journal of Biological Chemistry
Kuo-Hsiang Tang, Robert E Blankenship

Abstract

The anoxygenic green sulfur bacteria (GSBs) assimilate CO(2) autotrophically through the reductive (reverse) tricarboxylic acid (RTCA) cycle. Some organic carbon sources, such as acetate and pyruvate, can be assimilated during the phototrophic growth of the GSBs, in the presence of CO(2) or HCO(3)(-). It has not been established why the inorganic carbonis required for incorporating organic carbon for growth and how the organic carbons are assimilated. In this report, we probed carbon flux during autotrophic and mixotrophic growth of the GSB Chlorobaculum tepidum. Our data indicate the following: (a) the RTCA cycle is active during autotrophic and mixotrophic growth; (b) the flux from pyruvate to acetyl-CoA is very low and acetyl-CoA is synthesized through the RTCA cycle and acetate assimilation; (c) pyruvate is largely assimilated through the RTCA cycle; and (d) acetate can be assimilated via both of the RTCA as well as the oxidative (forward) TCA (OTCA) cycle. The OTCA cycle revealed herein may explain better cell growth during mixotrophic growth with acetate, as energy is generated through the OTCA cycle. Furthermore, the genes specific for the OTCA cycle are either absent or down-regulated during phototrophic growth, implyin...Continue Reading

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Citations

Oct 16, 2012·Extremophiles : Life Under Extreme Conditions·Lilia MontoyaAngel G Alpuche-Solís
Feb 26, 2013·Photosynthesis Research·Joseph Kuo-Hsiang TangSun W Tam
Oct 13, 2010·The Journal of Biological Chemistry·Xueyang FengYinjie J Tang
Jun 2, 2014·Photosynthesis Research·Yaya WangJoseph Kuo-Hsiang Tang
Nov 6, 2012·Biotechnology and Bioengineering·Jonathan P BadalamentiRosa Krajmalnik-Brown
Sep 21, 2013·Biophysical Journal·Joseph Kuo-Hsiang TangAlán Aspuru-Guzik
Oct 12, 2018·Environmental Microbiology·Anna P FlorentinoIrene Sánchez-Andrea
Jun 16, 2012·The Journal of Pharmacology and Experimental Therapeutics·Robert K Naviaux
Jun 17, 2020·Genome Biology and Evolution·Aurélien SaghaïPurificación López-García
May 15, 2018·Frontiers in Microbiology·Sebastian HaasJennifer L Macalady
Sep 3, 2010·The Journal of Biological Chemistry·Kuo-Hsiang TangYinjie J Tang
Oct 12, 2017·Photosynthesis Research·Bob B BuchananIvan A Berg
Nov 22, 2018·Frontiers in Cellular and Infection Microbiology·Garima AroraRamandeep Singh

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