Both glypican-3/Wnt/β-catenin signaling pathway and autophagy contributed to the inhibitory effect of curcumin on hepatocellular carcinoma

Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
Pei HuZhongji Meng

Abstract

The aim of this study is to investigate the role of glypican-3(GPC3)/wnt/β-catenin signaling pathway and autophagy in the regulation of hepatocellular carcinoma (HCC) growth mediated by curcumin. HepG2 cells were treated with various concentrations of curcumin and/or GPC3-targeting siRNA in the presence or absence of 3-MA. Cell proliferation and apoptosis were determined by MTT and TUNEL assay, respectively. Expression of GPC3, β-catenin, c-myc, LC3, and Beclin1 was determined by western blotting. In addition, curcumin was tested in tumor xenografts mice model, Caliper IVIS Lumina II was used to monitor the tumor growth, and GPC3/wnt/β-catenin signaling proteins were determined by western blotting. Curcumin treatment led to proliferation inhibition and apoptosis induction in HepG2 cells in a concentration-dependent manner, and suppressed HCC tumor growth in vivo. Further analysis showed that curcumin treatment inactivated Wnt/β-catenin signaling and decreased GPC3 expression, silencing of GPC3 expression promoted the effects of curcumin on Wnt/β-catenin signaling. In addition, inhibiting autophagy by 3-MA relieved curcumin-dependent down-regulation of GPC3. Curcumin suppressed HCC tumor growth through down-regulating GPC3/wnt/β...Continue Reading

Citations

Dec 5, 2019·World Journal of Gastrointestinal Oncology·Zi-Lin HuangLu-Jun Shen
Sep 16, 2020·International Journal of Molecular Sciences·Monica BenvenutoRoberto Bei
Dec 15, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Shahrokh LorzadehNegar Azarpira
Mar 7, 2020·Current Pharmaceutical Biotechnology·Amirhossein SahebkarHossein M Orafai
Jun 15, 2021·Frontiers in Molecular Biosciences·Qianhui XuWen Huang
Jun 27, 2021·Phytotherapy Research : PTR·Korosh MorshediHamed Mirzaei
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Qianhui XuWen Huang
Aug 31, 2021·Signal Transduction and Targeted Therapy·Fanyuan YuLing Ye

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