Both haemagglutinin-specific antibody and T cell responses induced by a chimpanzee adenoviral vaccine confer protection against influenza H7N9 viral challenge

Scientific Reports
Xiang WangDongming Zhou

Abstract

Since 2013, the outbreak or sporadic infection of a new reassortant H7N9 influenza virus in China has resulted in hundreds of deaths and thousands of illnesses. An H7N9 vaccine is urgently needed, as a licensed human vaccine against H7N9 influenza is currently not available. Here, we developed a recombinant adenovirus-based vaccine, AdC68-H7HA, by cloning the H7N9 haemagglutinin (HA) gene into the chimpanzee adenoviral vector AdC68. The efficacy of AdC68-H7HA was evaluated in mice as well as guinea pigs. For comparison, an H7N9 DNA vaccine based on HA was also generated and tested in mice and guinea pigs. The results demonstrated that both AdC68-H7HA and the DNA vaccine prime-adenovirus boost regimen induced potent immune responses in animals and completely protected mice from lethal H7N9 influenza viral challenge. A post-immunization serum transfer experiment showed that antibody responses could completely protect against lethal challenge, while a T cell depletion experiment indicated that HA-specific CD8+ T cells responses also contributed to protection. Therefore, both HA-specific humoral immunity and cellular immunity play important roles in the protection. These data suggest that the chimpanzee adenovirus expressing HA is ...Continue Reading

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Citations

Aug 23, 2019·Chemical Biology & Drug Design·Jiangfei ZhouChen Wang
Dec 21, 2019·Journal of Peptide Science : an Official Publication of the European Peptide Society·Jiangfei ZhouChen Wang
Feb 23, 2018·Frontiers in Microbiology·Daniela S Rajão, Daniel R Pérez
Feb 27, 2021·Frontiers in Immunology·Lucas J KerstetterLynda Coughlan

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Datasets Mentioned

BETA
KJ633809.1

Methods Mentioned

BETA
flow cytometry
PCRs
density-gradient centrifugation
FACS
serum collection
ELISAs
ELISA
reverse transcription-PCR

Software Mentioned

FlowJo
GraphPad Prism
REALPLEX2
Tree

Related Concepts