PMID: 9436615Jan 22, 1998Paper

Both hydroxylamine and nitroxide protect cardiomyocytes from oxidative stress

Free Radical Biology & Medicine
R ZhangAyelet M Samuni

Abstract

The unique anti-oxidative activity of nitroxide radicals protecting against reactive oxygen-derived species (ROS) has been recently demonstrated in several model systems. The present study focuses on the activity of nitroxide and of its reduced form in cultured rat ventricular cardiomyocytes exposed to O2.- and H2O2 generated by hypoxanthine (HX) and xanthine oxidase (XO). To evaluate cell injury, spontaneous beating, leakage of lactate dehydrogenase (LDH), and depletion of cellular ATP were determined. The protective effect of 4-OH-2,2,6,6-tetramethyl-piperidine-N-oxyl (TPL) was compared with that of 4-OH-2,2,6,6-tetramethyl-1-hydroxypiperidine (TPL-H) and of several common anti-oxidants. A rapid exchange between TPL and TPL-H, is mediated by cellular metabolism and through reactions with ROS. In particular, TPL under O2.- flux is oxidized to oxo-ammonium cation (TPL+) which comproportionates with TPL-H yielding two nitroxide radicals. Because this exchange limits the distinction between the biological activities of TPL and TPL-H, NADH which can reduce TPL+ was included in order to maintain the nitroxide in its reduced form. The results demonstrate that both TPL and TPL-H protect cardiomyocytes against beating loss and LDH lea...Continue Reading

References

Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D GelvanS R Powell
Jan 1, 1991·Free Radical Research Communications·A SamuniA Russo
Aug 15, 1991·Archives of Biochemistry and Biophysics·J B MitchellA Russo
Dec 1, 1991·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·H M SwartzK Hideg
Jan 15, 1991·Biochemistry·A SamuniJ B Mitchell
May 1, 1991·The Journal of Clinical Investigation·A SamuniA Russo
Aug 1, 1991·Journal of Molecular and Cellular Cardiology·A Beresewicz, M Horackova
Mar 20, 1990·Biochemistry·J B MitchellA Russo
Jan 1, 1989·Free Radical Biology & Medicine·A SamuniA Russo
Jan 1, 1989·Free Radical Biology & Medicine·U A NilssonA C Bylund-Fellenius
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W FronciszJ S Hyde
Feb 2, 1987·Life Sciences·E G AnkelZ Zivkovic
Jan 1, 1987·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·D J ChambersD J Hearse
Jul 1, 1988·Journal of the American College of Cardiology·R Bolli
Dec 1, 1987·The Journal of Clinical Investigation·J L ZweierM L Weisfeldt
Apr 26, 1995·Molecular and Cellular Biochemistry·M MohsenA Samuni
Jan 1, 1994·Methods in Enzymology·M C Krishna, A Samuni
Jan 1, 1994·The American Journal of Physiology·D R JaneroH M Sharif
Oct 18, 1996·The Journal of Biological Chemistry·M C KrishnaA Samuni
Oct 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·F WROBLEWSKI, J S LADUE

❮ Previous
Next ❯

Citations

Nov 26, 2003·European Journal of Pharmacology·Ayotunde S O AdeagboPaul J Matheson
Jul 11, 2002·Life Sciences·Sunday O Awe, Ayotunde S O Adeagbo
Nov 26, 2002·Free Radical Biology & Medicine·Nimesh S A PatelChristoph Thiemermann
Nov 13, 2004·The Journal of Nutritional Biochemistry·W R LeifertE J McMurchie
Mar 21, 2003·Vascular Pharmacology·Sunday O AweAyotunde S O Adeagbo
Dec 9, 2009·The Journal of Physical Chemistry. a·Frederick A Villamena
Jun 20, 2012·Future Medicinal Chemistry·Frederick A VillamenaKevin M Nash
Jan 22, 2008·Free Radical Biology & Medicine·Jan TrnkaMichael P Murphy
May 28, 2008·Free Radical Biology & Medicine·Sonia DonzelliDavid A Wink
Aug 15, 2015·Environmental Science & Technology·Liwei ChenJun Ma
Feb 11, 2014·Journal of Enzyme Inhibition and Medicinal Chemistry·Gennaro TaibiGiuseppe Carruba
Oct 7, 2016·Cell Biochemistry and Biophysics·Christopher Prescott, Steven E Bottle
Mar 17, 2007·Free Radical Research·Ileana Dragutan, Rolf J Mehlhorn
Nov 23, 2017·International Journal of Molecular Sciences·Marcin Lewandowski, Krzysztof Gwozdzinski
Dec 6, 2008·Free Radical Research·Jan TrnkaMichael P Murphy
Sep 1, 2020·Free Radical Biology & Medicine·Amram Samuni, Sara Goldstein
Nov 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jan CzepasKrzysztof Gwoździński

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.